FLRT2 elisa kit

Rat FLRT2 PicoKine ELISA Kit

Cat. Num. AAA1751715

• Reactivity: Rat; There is no detectable cross-reactivity with other relevant proteins.
• Synonyms: FLRT2; Rat FLRT2 PicoKine ELISA Kit; Rat FLRT2; Leucine-rich repeat transmembrane protein FLRT2; Fibronectin-like domain-containing leucine-rich transmembrane protein 2; Flrt2; Fibronectin leucine rich transmembrane protein 2; FLRT2 elisa kit

• Reactivity: Rat; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant Rat Fgfbp1
• Sequence Length: 660

• Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta)
• Detection Range: 78pg/ml-5000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months.; Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FLRT2 elisa kit

Principle of the Assay: The Picokine Rat Fgfbp1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Fgfbp1 with a 96-well strip plate that is pre-coated with antibody specific for Fgfbp1. The detection antibody is a biotinylated antibody specific for Fgfbp1. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Rat Fgfbp1 with immunogen: Expression system for standard: NSO, Immunogen sequence: E21-C238. The kit is analytically validated with ready to use reagents. To measure Rat Fgfbp1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Rat Fgfbp1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Fgfbp1 in the sample!!Background/Introduction: Fibroblast growth factor-binding protein 1 is a protein that in humans is encoded by the FGFBP1 gene. This gene encodes a secreted fibroblast growth factor carrier protein. The encoded protein plays a critical role in cell proliferation, differentiation and migration by binding to fibroblast growth factors and potentiating their biological effects on target cells. The encoded protein may also play a role in tumor growth as an angiogenic switch molecule, and expression of this gene has been associated with several types of cancer including pancreatic and colorectal adenocarcinoma. A pseudogene of this gene is also located on the short arm of chromosome 5.

NCBI and Uniprot Product Information

• NCBI GI #: 157822675
• NCBI GeneID: 299236
• NCBI Accession #: NP_001100220.1
• NCBI GenBank Nucleotide #: NM_001106750.1
• UniProt Accession #: D3ZTV3
• Molecular Weight: 73,874 Da
• NCBI Official Full Name: leucine-rich repeat transmembrane protein FLRT2
• NCBI Official Synonym Full Names: fibronectin leucine rich transmembrane protein 2
• NCBI Official Symbol: Flrt2
• NCBI Protein Information: leucine-rich repeat transmembrane protein FLRT2
• UniProt Protein Name: Leucine-rich repeat transmembrane protein FLRT2
• Protein Family: Leucine-rich repeat transmembrane protein
• UniProt Gene Name: Flrt2

Uniprot Description

Functions in cell-cell adhesion, cell migration and axon guidance. Mediates cell-cell adhesion via its interactions with ADGRL3 and probably also other latrophilins that are expressed at the surface of adjacent cells. May play a role in the migration of cortical neurons during brain development via its interaction with UNC5D. Mediates axon growth cone collapse and plays a repulsive role in neuron guidance via its interaction with UNC5D, and possibly also other UNC-5 family members. Plays a role in fibroblast growth factor-mediated signaling cascades. Required for normal organization of the cardiac basement membrane during embryogenesis, and for normal embryonic epicardium and heart morphogenesis.