FLRT3 elisa kit

Human FLRT3 PicoKine ELISA Kit

Cat. Num. AAA1751716

• Gene Names: FLRT3; HH21
• Reactivity: Human; There is no detectable cross-reactivity with other relevant proteins.
• Synonyms: FLRT3; Human FLRT3 PicoKine ELISA Kit; Human FLRT3; Leucine-rich repeat transmembrane protein FLRT3; Fibronectin-like domain-containing leucine-rich transmembrane protein 3; KIAA1469; UNQ856/PRO1865; Fibronectin leucine rich transmembrane protein 3; FLRT3 elisa kit

• Reactivity: Human; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant Human FLRT2
• Sequence Length: 649

• Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta)
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months.; Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FLRT3 elisa kit

Principle of the Assay: The Picokine Human FLRT2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human FLRT2 with a 96-well strip plate that is pre-coated with antibody specific for FLRT2. The detection antibody is a biotinylated antibody specific for FLRT2. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Human FLRT2 with immunogen: Expression system for standard: NSO, Immunogen sequence: C36-T660. The kit is analytically validated with ready to use reagents. To measure Human FLRT2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human FLRT2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human FLRT2 in the sample!!Background/Introduction: Fibronectin leucine-rich repeat transmembrane protein FLRT2 is a protein that in humans is encoded by the FLRT2 gene. It is mapped to 14q31.3. This gene encodes a member of the fibronectin leucine rich transmembrane (FLRT) family of cell adhesion molecules, which regulate early embryonic vascular and neural development. The encoded type I transmembrane protein has an extracellular region consisting of an N-terminal leucine-rich repeat domain and a type 3 fibronectin domain, followed by a transmembrane domain and a short C-terminal cytoplasmic tail domain. It functions as both a homophilic cell adhesion molecule and a heterophilic chemorepellent through its interaction with members of the uncoordinated-5 receptor family. Proteolytic removal of the extracellular region controls the migration of neurons in the developing cortex. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 7019383
• NCBI GeneID: 23767
• NCBI Accession #: NP_037413.1
• NCBI GenBank Nucleotide #: NM_013281.3
• UniProt Accession #: Q9NZU0; Q542Z9; Q96K39; Q96K42; Q96KB1; Q9P259; D3DW20
• Molecular Weight: 73,004 Da
• NCBI Official Full Name: leucine-rich repeat transmembrane protein FLRT3
• NCBI Official Synonym Full Names: fibronectin leucine rich transmembrane protein 3
• NCBI Official Symbol: FLRT3
• NCBI Official Synonym Symbols: HH21
• NCBI Protein Information: leucine-rich repeat transmembrane protein FLRT3
• UniProt Protein Name: Leucine-rich repeat transmembrane protein FLRT3
• Protein Family: Leucine-rich repeat transmembrane protein
• UniProt Gene Name: FLRT3
• UniProt Synonym Gene Names: KIAA1469

NCBI Description

This gene encodes a member of the fibronectin leucine rich transmembrane protein (FLRT) family. FLRTs may function in cell adhesion and/or receptor signalling. Their protein structures resemble small leucine-rich proteoglycans found in the extracellular matrix. This gene is expressed in many tissues. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

Functions in cell-cell adhesion, cell migration and axon guidance, exerting an attractive or repulsive role depending on its interaction partners. Plays a role in the spatial organization of brain neurons. Plays a role in vascular development in the retina (). Plays a role in cell-cell adhesion via its interaction with ADGRL3 and probably also other latrophilins that are expressed at the surface of adjacent cells (PubMed:26235030). Interaction with the intracellular domain of ROBO1 mediates axon attraction towards cells expressing NTN1. Mediates axon growth cone collapse and plays a repulsive role in neuron guidance via its interaction with UNC5B, and possibly also other UNC-5 family members (). Promotes neurite outgrowth (in vitro) (PubMed:14706654). Mediates cell-cell contacts that promote an increase both in neurite number and in neurite length. Plays a role in the regulation of the density of glutamaergic synapses. Plays a role in fibroblast growth factor-mediated signaling cascades. Required for normal morphogenesis during embryonic development, but not for normal embryonic patterning. Required for normal ventral closure, headfold fusion and definitive endoderm migration during embryonic development. Required for the formation of a normal basement membrane and the maintenance of a normal anterior visceral endoderm during embryonic development ().