FLRT2 elisa kit

Mouse FLRT2 PicoKine ELISA Kit

Cat. Num. AAA1751714

• Reactivity: Mouse; There is no detectable cross-reactivity with other relevant proteins.
• Synonyms: FLRT2; Mouse FLRT2 PicoKine ELISA Kit; Mouse FLRT2; Leucine-rich repeat transmembrane protein FLRT2; Fibronectin-like domain-containing leucine-rich transmembrane protein 2; Flrt2; Kiaa0405; Fibronectin leucine rich transmembrane protein 2; FLRT2 elisa kit

• Reactivity: Mouse; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant Mouse Flrt2
• Sequence Length: 660

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10 pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months.; Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FLRT2 elisa kit

Principle of the Assay: The Mouse Flrt2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Flrt2 with a 96-well strip plate that is pre-coated with antibody specific for Flrt2. The detection antibody is a biotinylated antibody specific for Flrt2. The capture antibody is A monoclonal antibody from rat, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Mouse Flrt2 with immunogen: Expression system for standard: NSO, Immunogen sequence: C36-T660. The kit is analytically validated with ready to use reagents. To measure Mouse Flrt2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Flrt2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Flrt2 in the sample.!!Background/Introduction: Fibronectin leucine-rich repeat transmembrane protein FLRT2 is a protein that in humans is encoded by the FLRT2 gene. It is mapped to 12; 12 E. This gene encodes a member of the fibronectin leucine rich transmembrane (FLRT) family of cell adhesion molecules, which regulate early embryonic vascular and neural development. The encoded type I transmembrane protein has an extracellular region consisting of an N-terminal leucine-rich repeat domain and a type 3 fibronectin domain, followed by a transmembrane domain and a short C-terminal cytoplasmic tail domain. It functions as both a homophilic cell adhesion molecule and a heterophilic chemorepellent through its interaction with members of the uncoordinated-5 receptor family. Proteolytic removal of the extracellular region controls the migration of neurons in the developing cortex. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 41462393
• NCBI GeneID: 399558
• NCBI Accession #: NP_958926.1
• NCBI GenBank Nucleotide #: NM_201518.4
• UniProt Accession #: Q8BLU0; Q6A073
• Molecular Weight: 73,948 Da
• NCBI Official Full Name: leucine-rich repeat transmembrane protein FLRT2
• NCBI Official Synonym Full Names: fibronectin leucine rich transmembrane protein 2
• NCBI Official Symbol: Flrt2
• NCBI Protein Information: leucine-rich repeat transmembrane protein FLRT2
• UniProt Protein Name: Leucine-rich repeat transmembrane protein FLRT2
• Protein Family: Leucine-rich repeat transmembrane protein
• UniProt Gene Name: Flrt2

Uniprot Description

Functions in cell-cell adhesion, cell migration and axon guidance. Mediates cell-cell adhesion via its interactions with ADGRL3 and probably also other latrophilins that are expressed at the surface of adjacent cells (PubMed:21350012, PubMed:25728924 PubMed:25374360). May play a role in the migration of cortical neurons during brain development via its interaction with UNC5D (PubMed:21673655). Mediates axon growth cone collapse and plays a repulsive role in neuron guidance via its interaction with UNC5D, and possibly also other UNC-5 family members (PubMed:21673655, PubMed:25728924). Plays a role in fibroblast growth factor-mediated signaling cascades (PubMed:16872596). Required for normal organization of the cardiac basement membrane during embryogenesis, and for normal embryonic epicardium and heart morphogenesis (PubMed:21350012).