Mouse FABP2 ELISA Kit | FABP2 elisa kit

Mouse FABP2 PicoKine ELISA Kit

Cat. Num. AAA1751561

• Gene Names: FABP2; FABPI; I-FABP
• Reactivity: Mouse
• Synonyms: FABP2; Mouse FABP2 PicoKine ELISA Kit; Mouse FABP2; FABP2 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Efemp1
• Sequence Length: 132

• Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta)
• Detection Range: 0.312ng/ml-20ng/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FABP2 elisa kit

Principle of the Assay: The Picokine Mouse Efemp1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Efemp1 with a 96-well strip plate that is pre-coated with antibody specific for Efemp1. The detection antibody is a biotinylated antibody specific for Efemp1. The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Mouse Efemp1 with immunogen: Expression system for standard: CHO, Immunogen sequence: Q18-F493. The kit is analytically validated with ready to use reagents. To measure Mouse Efemp1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Mouse Efemp1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Efemp1 in the sample!!Background/Introduction: EGF-containing fibulin-like extracellular matrix protein 1 is a protein that in humans is encoded by the EFEMP1 gene. This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.

NCBI and Uniprot Product Information

• NCBI GI #: 194097325
• NCBI GeneID: 2169
• NCBI Accession #: NP_000125.2
• NCBI GenBank Nucleotide #: NM_000134.3
• UniProt Accession #: P12104
• NCBI Official Full Name: fatty acid-binding protein, intestinal
• NCBI Official Synonym Full Names: fatty acid binding protein 2
• NCBI Official Symbol: FABP2
• NCBI Official Synonym Symbols: FABPI; I-FABP
• NCBI Protein Information: fatty acid-binding protein, intestinal
• Protein Family: Fatty acid-binding protein

NCBI Description

The protein encoded by this gene is an intracellular fatty acid-binding protein that participates in the uptake, intracellular metabolism, and transport of long-chain fatty acids. The encoded protein is also involved in the modulation of cell growth and proliferation. This protein binds saturated long-chain fatty acids with high affinity, and may act as a lipid sensor to maintain energy homeostasis. [provided by RefSeq, Aug 2017]