Human FABP2/I-FABP ELISA Kit | FABP2 elisa kit

Human FABP2/I-FABP PicoKine ELISA Kit

Cat. Num. AAA178728

• Gene Names: FABP2; FABPI; I-FABP
• Reactivity: Human
• Synonyms: FABP2/I-FABP; Human FABP2/I-FABP PicoKine ELISA Kit; FABP2; FABP 2; FABPI_HUMAN; Fatty acid-binding protein; Fatty acid-binding protein 2; I-FABP; intestinal; Intestinal-type fatty acid-binding protein; P12104; FABP2 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human FABP2
• Sequence Length: 132

• Samples: Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess interassay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FABP2 elisa kit

Principle of the Assay: The Picokine™ Human FABP2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human FABP2 with a 96-well strip plate that is pre-coated with antibody specific for FABP2. The detection antibody is a biotinylated antibody specific for FABP2. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human FABP2 with immunogen: Expression system for standard: E.coli; Immunogen sequence: A2-D132. The kit is analytically validated with ready to use reagents. To measure Human FABP2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human FABP2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human FABP2 in the sample.!!Background/Introduction: Fatty acid-binding protein 2 (FABP2), also known as Intestinal-type fatty acid-binding protein (I-FABP) is a protein that in humans is encoded by the FABP2 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. Using a human cDNA probe for in situ hybridization studies, they regionalized the assignment to 4q28-q31. The intestinal form of FABP was mapped to mouse chromosome 3 between the amylase and alcohol dehydrogenase-3 loci. Also, they may be responsible in the modulation of cell growth and proliferation.

NCBI and Uniprot Product Information

• NCBI GI #: 194097325
• NCBI GeneID: 2169
• NCBI Accession #: NP_000125.2
• NCBI GenBank Nucleotide #: NM_000134.3
• UniProt Accession #: P12104; Q2NKJ1
• Molecular Weight: 15,207 Da
• NCBI Official Full Name: fatty acid-binding protein, intestinal
• NCBI Official Synonym Full Names: fatty acid binding protein 2
• NCBI Official Symbol: FABP2
• NCBI Official Synonym Symbols: FABPI; I-FABP
• NCBI Protein Information: fatty acid-binding protein, intestinal
• UniProt Protein Name: Fatty acid-binding protein, intestinal
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: FABP2
• UniProt Synonym Gene Names: FABPI; I-FABP

NCBI Description

The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance. [provided by RefSeq, Jul 2008]

Uniprot Description

FABP2: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long- chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding; Transporter

Chromosomal Location of Human Ortholog: 4q26

Cellular Component: cytosol

Molecular Function: protein binding

Biological Process: digestion; triacylglycerol catabolic process