Typical Testing Data/Standard Curve
Cholic Acid ELISA Kit
Cholic Acid ELISA Kit
Synonyms
Cholic Acid; Cholic Acid ELISA Kit; Cholic Acid elisa kit
Assay Type
Competitive
Preparation and Storage
Upon receipt, aliquot and store both the Anti-Cholic Acid Antibody and 100X Cholic Acid Conjugate at -20 degree C and avoid multiple freeze/thaw cycles
Store all other components at 4 degree C.
Store all other components at 4 degree C.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for Cholic Acid elisa kit
Principle of the Assay||The Cholic Acid ELISA kit is a competitive ELISA for the quantitative measurement of cholic acid. The unknown cholic acid samples or cholic acid standards are first added to a Cholic Acid Conjugate preadsorbed microplate. After a brief incubation, an Anti-Cholic Acid monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The cholic acid content in unknown samples is determined by comparison with predetermined cholic acid standard curve.
Background/Introduction: Cholic acid is a primary bile acid. Along with chenodeoxycholic acid, cholic acid is one of the two major bile acids synthesized from cholesterol by the liver. Bile is a complex mixture of lipids, protein, carbohydrates, mineral salts, vitamins, and various trace elements, with bile acids making up about 67% of the total composition. Bile acids are produced from excess cholesterol, secreted from the liver, absorbed into the small intestines, and returned to the liver with portal blood. While bile acid synthesis is critical for the removal of cholesterol from the body, bile acids are also needed for proper uptake of dietary lipids, fat soluble vitamins, and other nutrients into the small intestines. Under physiological conditions, newly synthesized bile acids are conjugated to glycine or taurine to form bile salts, and not much free bile acid is actually found in bile. Determining circulatory levels of bile acids can be used to identify or identify certain liver diseases. In addition, elevated serum bile levels have been observed in intrahepatic cholestasis of pregnancy cases. Recently, bile acids have been recognized as integrators of metabolic processes as well as signaling molecules. The Cholic Acid ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of cholic acid in serum, feces, or other cell or tissue samples. The quantity of cholic acid in unknown samples is determined by comparing its absorbance with that of a known cholic acid standard curve. The kit has detection sensitivity limit of 0.4 uM cholic acid. Each Cholic Acid ELISA Kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.
Background/Introduction: Cholic acid is a primary bile acid. Along with chenodeoxycholic acid, cholic acid is one of the two major bile acids synthesized from cholesterol by the liver. Bile is a complex mixture of lipids, protein, carbohydrates, mineral salts, vitamins, and various trace elements, with bile acids making up about 67% of the total composition. Bile acids are produced from excess cholesterol, secreted from the liver, absorbed into the small intestines, and returned to the liver with portal blood. While bile acid synthesis is critical for the removal of cholesterol from the body, bile acids are also needed for proper uptake of dietary lipids, fat soluble vitamins, and other nutrients into the small intestines. Under physiological conditions, newly synthesized bile acids are conjugated to glycine or taurine to form bile salts, and not much free bile acid is actually found in bile. Determining circulatory levels of bile acids can be used to identify or identify certain liver diseases. In addition, elevated serum bile levels have been observed in intrahepatic cholestasis of pregnancy cases. Recently, bile acids have been recognized as integrators of metabolic processes as well as signaling molecules. The Cholic Acid ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of cholic acid in serum, feces, or other cell or tissue samples. The quantity of cholic acid in unknown samples is determined by comparing its absorbance with that of a known cholic acid standard curve. The kit has detection sensitivity limit of 0.4 uM cholic acid. Each Cholic Acid ELISA Kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.
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Product Notes
The Cholic Acid (Catalog #AAA169246) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Cholic Acid, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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