Testing Data
OxiSelect Comet Assay Slides (3-Well) Accessory Reagent
OxiSelect Comet Assay Slides (3-Well)
Synonyms
OxiSelect Comet Assay Slides (3-Well); OxiSelect Comet Assay Slides (3-Well) accessory reagent
Application Notes
OxiSelect 3-Well Comet Assay Slides are specially treated for the adhesion of low-melting agarose used in the comet assay. These slides may be used in conjunction with reagents found in our OxiSelect Comet Assay Kit or with your own comet assay reagents.
Preparation and Storage
STORAGE: Room Temperature
Testing Data
Testing Data
Related Product Information for OxiSelect Comet Assay Slides (3-Well) accessory reagent
Background: DNA damage, due to environmental factors and normal metabolic processes inside the cell, occurs at a rate of 1,000 to 1,000,000 molecular lesions per cell per day. While this counts for only a small part of the human genome's approximately 6 billion bases (3 billion base pairs), unrepaired lesions to critical genes can impede a cell's ability to carry out its function and appreciably increase the likelihood of cancer. The comet assay, or single cell gel electrophoresis assay (SCGE), is a common technique for measurement of DNA damage in individual cells. Under an electrophoretic field, damaged cellular DNA (containing fragments and strand breaks) is separated from intact DNA, yielding a classic "comet tail" shape under the microscope. Extent of DNA damage is usually visually estimated by comet tail measurement; however, image analysis software is also available for measuring various parameters.
Product Categories/Family for OxiSelect Comet Assay Slides (3-Well) accessory reagent
References
1. Ostling, O., and Johanson, K. J. (1984). Micro gel electrophoretic study of radiation induced DNA damages in individual mammalian cells. Biochem. Biophys. Res. Commun. 123, 291-298.
2. Singh, N. P., McCoy, M. T., Tice, R. R., and Schneider, E. L. (1988). A simple technique for quantification of low levels of DNA damage in individual cells. Exp. Cell. Res. 175, 184-191.
3. Olive, P. L., Banath, J. P., and Durand, R. E. (1990a). Heterogeneity in radiation induced DNA damage and repair in tumor and normal cells using the "Comet" assay. Radiat. Res. 122, 86-94.
4. De Boeck, M., Touil, N., De Visscher, G., Vande, P. A., and Kirsch-Volders, M. (2000). Validation and implementation of an internal standard in Comet assay. Mutat. Res. 469, 181-197.
Liu, L. et al. (2014). TGFbeta Induces "BRCAness" and Sensitivity to PARP Inhibition in Breast Cancer by Regulating DNA-Repair Genes. Mol Cancer Res. 12:1597-1609.
2. Singh, N. P., McCoy, M. T., Tice, R. R., and Schneider, E. L. (1988). A simple technique for quantification of low levels of DNA damage in individual cells. Exp. Cell. Res. 175, 184-191.
3. Olive, P. L., Banath, J. P., and Durand, R. E. (1990a). Heterogeneity in radiation induced DNA damage and repair in tumor and normal cells using the "Comet" assay. Radiat. Res. 122, 86-94.
4. De Boeck, M., Touil, N., De Visscher, G., Vande, P. A., and Kirsch-Volders, M. (2000). Validation and implementation of an internal standard in Comet assay. Mutat. Res. 469, 181-197.
Liu, L. et al. (2014). TGFbeta Induces "BRCAness" and Sensitivity to PARP Inhibition in Breast Cancer by Regulating DNA-Repair Genes. Mol Cancer Res. 12:1597-1609.
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Product Notes
The OxiSelect Comet Assay Slides (3-Well) (Catalog #AAA168753) is an Accessory Reagent and is intended for research purposes only. The product is available for immediate purchase. OxiSelect 3-Well Comet Assay Slides are specially treated for the adhesion of low-melting agarose used in the comet assay. These slides may be used in conjunction with reagents found in our OxiSelect Comet Assay Kit or with your own comet assay reagents. Researchers should empirically determine the suitability of the OxiSelect Comet Assay Slides (3-Well) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OxiSelect Comet Assay Slides (3-Well), Accessory Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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