SDS-Page
(Recombinant YEATS2 (200-340) protein gel 12.5% SDS-PAGE Coomassie staining MW: 21.5 kDa Purity: >80% )
YEATS2 recombinant protein
Recombinant YEATS2 (200-340) protein
Synonyms
YEATS2; Recombinant YEATS2 (200-340) protein; YEATS Domain Containing 2; YEATS2 recombinant protein
Host
E Coli
Form/Format
Recombinant YEATS2 (200-340) protein is supplied in 25mM Tris-HCl pH8.0, 300mM NaCl, 10% glycerol and 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Notes
Recombinant YEATS2 (200-340) protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling. Binding Assay Conditions: 3uM H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of YEATS2 (200-340) protein in a 10ul reaction system containing 50mM HEPES-NaOH pH7.5, 0.1% BSA for 1 hour, then 10ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant YEATS2 (200-340) protein gel 12.5% SDS-PAGE Coomassie staining MW: 21.5 kDa Purity: >80% )
SDS-Page
(Recombinant YEATS2 (200-340) protein gel 12.5% SDS-PAGE Coomassie staining MW: 21.5 kDa Purity: >80% )
Testing Data
(Recombinant YEATS2 (200-340) protein activity assay H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of YEATS2 (200-340) protein in reaction buffer for 1 hour, then DYKDDDDK antibody and SA-XL665 mixture was added to each reaction system for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(Recombinant YEATS2 (200-340) protein activity assay H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of YEATS2 (200-340) protein in reaction buffer for 1 hour, then DYKDDDDK antibody and SA-XL665 mixture was added to each reaction system for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(Recombinant YEATS2 (200-340) protein activity assay H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of YEATS2 (200-340) protein in reaction buffer for 1 hour, then DYKDDDDK antibody and SA-XL665 mixture was added to each reaction system for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(Recombinant YEATS2 (200-340) protein activity assay H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of YEATS2 (200-340) protein in reaction buffer for 1 hour, then DYKDDDDK antibody and SA-XL665 mixture was added to each reaction system for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Related Product Information for YEATS2 recombinant protein
Short Description: Recombinant human YEATS2 (200-340) includes amino acids 200-340 (accession number NP_001338299.1); it was expressed in E Coli with an N-terminal 6xHis-tag and a C-terminal DYKDDDDK tag. The molecular weight of the protein is 21.5 kDa. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: YEATS2 (YEATS Domain Containing 2), is a component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. YEATS2 specifically recognizes and binds histone H3 crotonylated at 'Lys-27' (H3K27cr). Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors.
Background: YEATS2 (YEATS Domain Containing 2), is a component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. YEATS2 specifically recognizes and binds histone H3 crotonylated at 'Lys-27' (H3K27cr). Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors.
Product Categories/Family for YEATS2 recombinant protein
NCBI and Uniprot Product Information
Molecular Weight
Molecular weight of the protein is 21.5kDa
Protein Family
Similar Products
Product Notes
The YEATS2 (Catalog #AAA389156) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "YEATS2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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