SDS-Page
(Recombinant MLLT3 / AF9 (1-138) protein, His / DYKDDDDK-Tag 10% SDS-PAGE Coomassie staining MW: 20.8 kDa Purity: >79%)
MLLT3/AF9 recombinant protein
Recombinant MLLT3/AF9 (1-138) protein, His/DYKDDDDK Tag
Shipping Temp: Dry Ice
SDS-Page
(Recombinant MLLT3 / AF9 (1-138) protein, His / DYKDDDDK-Tag 10% SDS-PAGE Coomassie staining MW: 20.8 kDa Purity: >79%)
SDS-Page
(Recombinant MLLT3 / AF9 (1-138) protein, His / DYKDDDDK-Tag 10% SDS-PAGE Coomassie staining MW: 20.8 kDa Purity: >79%)
Testing Data
(Recombinant MLLT3 / AF9 (1-138) protein, His / DYKDDDDK-Tag activity assay 3 uM H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of MLLT3 / AF9 (1-138) protein, in a 10 ul system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hr, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(Recombinant MLLT3 / AF9 (1-138) protein, His / DYKDDDDK-Tag activity assay 3 uM H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of MLLT3 / AF9 (1-138) protein, in a 10 ul system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hr, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(Recombinant MLLT3 / AF9 (1-138) protein, His / DYKDDDDK-Tag activity assay 3 uM H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of MLLT3 / AF9 (1-138) protein, in a 10 ul system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hr, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(Recombinant MLLT3 / AF9 (1-138) protein, His / DYKDDDDK-Tag activity assay 3 uM H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of MLLT3 / AF9 (1-138) protein, in a 10 ul system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hr, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Background: MLLT3 (Myeloid/Lymphoid Or Mixed-Lineage Leukemia; translocated To, 3), also known as AF9 or-YEATS3, is a component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. It specifically recognizes and binds acylated histone H3, with a marked preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones.
NCBI and Uniprot Product Information
Uniprot Description
MLLT3: A chromosomal aberration involving MLLT3 is associated with acute leukemias. Translocation t(9;11)(p22;q23) with MLL/HRX. The result is a rogue activator protein.
Protein type: Cell development/differentiation; Oncoprotein
Chromosomal Location of Human Ortholog: 9p22
Cellular Component: transcription elongation factor complex; nucleus
Molecular Function: protein binding
Biological Process: anterior/posterior pattern formation; segment specification; regulation of transcription, DNA-dependent; transcription, DNA-dependent
Research Articles on MLLT3/AF9
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Product Notes
The MLLT3/AF9 mllt3 (Catalog #AAA389247) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "MLLT3/AF9, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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