IGF1 active protein

IGF1 Protein, Mouse, Recombinant

Cat. Num. AAA8122741

• Gene Names: Igf1; Igf-1; Igf-I; C730016P09Rik
• Purity: >95% as determined by SDS-PAGE
• Synonyms: IGF1; IGF1 Protein; Mouse; Recombinant; Mouse IGF1/IGF?I/IGF-1 protein; C730016P09Rik Protein; Igf-1 Protein; Igf-I Protein; insulin-like growth factor 1 (somatomedin C); IGF1 active protein

• Host: Yeast
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gly49-Ala118

• Species: Mouse
• Activity: Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is 0.2-1.2ug/mL.
• Predicted N Terminal: Gly 49
• Tag: Native
• Protein Construction: A DNA sequence encoding the mouse IGF1 (NP_001104744.1)(Gly49-Ala118) was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is 0.2-1.2 ug/mL.)

Related Product Information for IGF1 active protein

Background: IGF I, also known as Mechano Growth Factor, somatomedin-C, IGF-I, and IGF1, is a secreted protein that belongs to the insulin family. The insulin family, comprised of insulin, relaxin, insulin-like growth factors I and II (IGF-I and IGF-II), and possibly the beta-subunit of 7S nerve growth factor, represents a group of structurally related polypeptides whose biological functions have diverged. The IGFs, or somatomedins, constitute a class of polypeptides that have a key role in pre-adolescent mammalian growth. IGF-I expression is regulated by GH and mediates postnatal growth, while IGF-II appears to be induced by placental lactogen during prenatal development. IGF1/IGF-I may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. IGF1/IGF-I stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also about enhancing glucose uptake. Defects in IGF1/IGF-I are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness, and mental retardation.

Product Categories/Family for IGF1 active protein

Serine Proteases and Regulators

References

Jansen M., et al.,(1983), Sequence of cDNA encoding human insulin-like growth factor I precursor. Nature 306:609-611.de Pagter-Holthuizen P., et al., (1986), Organization of the human genes for insulin-like growth factors I and II.FEBS Lett. 195:179-184.le Bouc Y., et al.,(1986), Complete characterization of the human IGF-I nucleotide sequence isolated from a newly constructed adult liver cDNA library.FEBS Lett. 196:108-112.

NCBI and Uniprot Product Information

• NCBI GI #: 163659889
• NCBI GeneID: 16000
• NCBI Accession #: NP_001104744.1
• NCBI GenBank Nucleotide #: NP_001104744.1
• UniProt Accession #: Q8CAR0; P05017
• Molecular Weight: 7.7
• NCBI Official Full Name: insulin-like growth factor I isoform 3
• NCBI Official Synonym Full Names: insulin-like growth factor 1
• NCBI Official Symbol: Igf1
• NCBI Official Synonym Symbols: Igf-1; Igf-I; C730016P09Rik
• NCBI Protein Information: insulin-like growth factor I
• UniProt Protein Name: Insulin-like growth factor I
• Protein Family: Insulin-like growth factor
• UniProt Gene Name: Igf1

NCBI Description

This gene encodes a member of the insulin-like growth factor (IGF) family of proteins that promote growth and development during fetal and postnatal life. This gene is predominantly expressed in the liver and the encoded protein undergoes proteolytic processing to generate a disulfide-linked mature polypeptide. Transgenic disruption of this gene in mice results in reduced postnatal survival and severe growth retardation. Mice lacking the encoded protein exhibit generalized organ hypoplasia including underdevelopment of the central nervous system and developmental defects in bone, muscle and reproductive systems. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]