IGFBP4 active protein

IGFBP4 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122637

• Gene Names: Igfbp4; Deb2; IGFBP-4; AI875747
• Purity: >95% as determined by SDS-PAGE
• Synonyms: IGFBP4; IGFBP4 Protein; Mouse; Recombinant (His Tag); Mouse IGFBP4 Protein (His Tag); AI875747 Protein; Deb2 Protein; IGFBP-4 Protein; insulin-like growth factor binding protein 4; IGFBP4 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu254

• Species: Mouse
• Activity: Measured in a serum free cell proliferation assay using MCF7 human breast adenocarcinoma cells. The ED50 for this effect is typically 0.1-0.6ug/ml
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Asp 22
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Glu 254) of mouse IGFBP4 (NP_034647.1) precursor was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for IGFBP4 active protein

Background: Insulin-like growth factor-binding protein 4 (IGFBP-4) is a 24-kDa protein that binds insulin-like growth factor 1 (IGF-1) and IGF-2 with high affinity and inhibits IGF action in vitro. The Insulin-like growth factor-binding protein also known as IGFBP serves as a carrier protein for Insulin-like growth factor 1. IGFBPs are distinct but are sharing regions with strong homology. All members of the IGFBP family bind IGF-I and IGF-II with about equal affinity. Insulin-like growth factor (IGF) binding proteins (IGFBPs) have been shown to either inhibit or enhance the action of IGF or act in an IGF-independent manner in the prostate. IGF-binding protein-4 (IGFBP-4) inhibits IGF-I action in vitro and is the most abundant IGFBP in the rodent arterial wall. Expression of IGFBP-4 mRNA in nontransgenic littermates was maximal in the liver and kidney. IGFBP-4 is a functional antagonist of IGF-I action on SMC. There is mounting evidence that the structure of the IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence, and IGF affinity.

References

Wang J, et al. (1998) Overexpression of insulin-like growth factor-binding protein-4 (IGFBP-4) in smooth muscle cells of transgenic mice through a smooth muscle alpha-actin-IGFBP-4 fusion gene induces smooth muscle hypoplasia. Endocrinology. 139(5): 2605-14.Chernausek SD, et al. (1995) Proteolytic cleavage of insulin-like growth factor binding protein 4 (IGFBP-4). Localization of cleavage site to non-homologous region of native IGFBP-4. J Biol Chem. 1995 May 12;270(19):11377-82. Karey, K.P. et al. 1988, Cancer Research 48: 4083.

NCBI and Uniprot Product Information

• NCBI GI #: 6981086
• NCBI GeneID: 16010
• NCBI Accession #: NP_034647.1
• NCBI GenBank Nucleotide #: NM_010517.3
• UniProt Accession #: P47879; O35666; Q3TMV0
• Molecular Weight: 27,807 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 4
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 4
• NCBI Official Symbol: Igfbp4
• NCBI Official Synonym Symbols: Deb2; IGFBP-4; AI875747
• NCBI Protein Information: insulin-like growth factor-binding protein 4
• UniProt Protein Name: Insulin-like growth factor-binding protein 4
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: Igfbp4
• UniProt Synonym Gene Names: Igfbp-4; IBP-4; IGF-binding protein 4; IGFBP-4

Uniprot Description

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.