Angiotensinogen Recombinant Protein | AGT recombinant protein

Angiotensinogen Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122693

• Gene Names: Agt; AngI; AngII; Aogen; AI265500; Serpina8
• Purity: >96% as determined by SDS-PAGE
• Synonyms: Angiotensinogen; Angiotensinogen Protein; Mouse; Recombinant (His Tag); Mouse Angiotensinogen/SerpinA8/AGT Protein (His Tag); AI265500 Protein; AngI Protein; AngII Protein; Aogen Protein; Serpina8 Protein; angiotensinogen (serpin peptidase inhibitor; clade A; member 8); AGT recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >96% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Val477

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Asp 25
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse AGT (P11859)(Met 1-Val 477) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for AGT recombinant protein

Background: Angiotensinogen, also known as AGT and SerpinA8, is a member of the serpin family. It is an alpha-2-globulin that is produced constitutively and released into the circulation mainly by the liver. Angiotensinogen is a essential component of the renin-angiotensin system (RAS) and a potent regulator of blood pressure. Angiotensinogen can be schematically considered to consist of a combination of an angiotensin I (Ang I) function, located at the N-terminal end, and the presence of a serpin (serine protease inhibitor) structure at the opposite end. Angiotensinogen is cleaved into three chains: Angiotensin-1 (Ang I), Angiotensin-2 (Ang II), and Angiotensin-3 (Ang III). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2. Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system. Defects in AGT are associated with susceptibility to essential hypertension and renal tubular dysgenesis (RTD). Several serpins (antithrombin, maspin, pigment epithelial-derived factor, and kallistatin) have been recently shown to exert an antiangiogenic activity, suggesting a common mechanism of endothelial cell proliferation and migration. Angiotensinogen/AGT and its renin-cleaved product, des(Ang I)AGT, are also angiogenesis inhibitors, both in vitro and in vivo at concentrations within the range of those observed in plasma. The Angiotensinogen products, that is angiotensin II and possibly angiotensin II-related products, have been found to act locally in modulating adipose tissue growth in an autocrine/paracrine manner. The transient or chronic overexpression of angiotensinogen in adipose tissue favors lipogenesis in adipocytes and leads to a 'vicious' circle whereby adipose tissue development is further increased.

Product Categories/Family for AGT recombinant protein

Serine Proteases and Regulators

References

Ailhaud G, et al. (2002) Angiotensinogen, adipocyte differentiation and fat mass enlargement. Curr Opin Clin Nutr Metab Care. 5(4): 385-9.Corvol P, et al. (2003) Inhibition of angiogenesis: a new function for angiotensinogen and des(angiotensin I)angiotensinogen. Curr Hypertens Rep. 5(2): 149-54.

NCBI and Uniprot Product Information

• NCBI GI #: 113881
• NCBI GeneID: 11606
• UniProt Accession #: P11859
• Molecular Weight: 51,990 Da
• NCBI Official Full Name: Angiotensinogen
• NCBI Official Synonym Full Names: angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• NCBI Official Symbol: Agt
• NCBI Official Synonym Symbols: AngI; AngII; Aogen; AI265500; Serpina8
• NCBI Protein Information: angiotensinogen; serpin A8; angiotensin I; angiotensin ll
• UniProt Protein Name: Angiotensinogen
• Protein Family: Angiotensinogen
• UniProt Gene Name: Agt
• UniProt Synonym Gene Names: Serpina8; Ang I; Ang II; Ang III; Ang IV
• UniProt Entry Name: ANGT_MOUSE

Uniprot Description

angiotensin: Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1- 8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2. Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT). Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause. Defects in AGT are a cause of renal tubular dysgenesis (RTD). RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Belongs to the serpin family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cytoplasm

Molecular Function: angiotensin receptor binding; serine-type endopeptidase inhibitor activity; sodium channel regulator activity; hormone activity; type 2 angiotensin receptor binding; type 1 angiotensin receptor binding

Biological Process: positive regulation of catalytic activity; extracellular matrix organization and biogenesis; renal system process; positive regulation of nitric oxide biosynthetic process; establishment of blood-nerve barrier; negative regulation of nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; positive regulation of multicellular organism growth; positive regulation of vasodilation; ovarian follicle rupture; regulation of apoptosis; positive regulation of fibroblast proliferation; cell surface receptor linked signal transduction; regulation of systemic arterial blood pressure by circulatory renin-angiotensin; positive regulation of superoxide release; negative regulation of neuron apoptosis; kidney development; angiotensin mediated regulation of renal output; response to muscle activity involved in regulation of muscle adaptation; regulation of calcium ion transport; regulation of norepinephrine secretion; brain renin-angiotensin system; negative regulation of tissue remodeling; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; phospholipase C activation; regulation of gene expression; angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure; regulation of transmission of nerve impulse; smooth muscle cell differentiation; cytokine secretion; positive regulation of blood pressure; regulation of long-term neuronal synaptic plasticity; peristalsis; cell-matrix adhesion; drinking behavior; renin-angiotensin regulation of aldosterone production; positive regulation of cellular protein metabolic process; smooth muscle cell proliferation; excretion; vasodilation; response to salt stress; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; fibroblast proliferation; regulation of blood pressure; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of cell proliferation; angiotensin mediated drinking behavior; artery smooth muscle contraction; blood vessel development; positive regulation of fatty acid biosynthetic process; vasoconstriction; cellular sodium ion homeostasis; renal response to blood flow during renin-angiotensin regulation of systemic arterial blood pressure; activation of NF-kappaB-inducing kinase; positive regulation of organ growth; positive regulation of peptidyl-serine phosphorylation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; ureteric bud branching; regulation of inflammatory response; hormone metabolic process; response to cold; negative regulation of cell growth; astrocyte activation