IGF1 active protein

Recombinant Mouse IGF1

Cat. Num. AAA4159974

• Gene Names: Igf1; Igf-1; Igf-I; C730016P09Rik
• Purity: >95% as determined by SDS-PAGE
• Synonyms: IGF1; Recombinant Mouse IGF1; IGF-I; Mechano growth factor; MGF; Somatomedin-C; IBP1; Recombinant Mouse Insulin Like Growth Factor-I (IGF1); IGF1 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 micron filtered solution in Phosphate buffer and NaCl
• Sequence Length: 153

• Bioactivity: The ED50 of Recombinant Mouse IGF1 as found by dose dependent proliferation of mouse 3T3 cells was found to be <0.1 ng/ml.
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method
• Reconstitution: A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Related Product Information for IGF1 active protein

The Insulin Like Growth Factors, or somatomedins, belong to the Insulin gene family, members of which are insulin and relaxin. The IGFs play similar a similar role to insulin with regards to the regulation of glucose uptake and glycogen metabolism. Additionally IGF1 plays an essential role in mediating many of the effects of growth hormone. IGFs are produced first as inactive precursors, and undergo proteolysis to become active. Circulating IGF1 is produced by hepatocytes whereas local IGF1 is produced in a variety of other tissues where it exhibits paracrine effects. Mouse IGF1 shares 94% and 98% amino acid sequence identity with human and rat IGF1 respectively. Recombinant Mouse IGF1 is a globular, 7.9 kDa protein which contains 3 native disulfide bonds.

NCBI and Uniprot Product Information

• NCBI GI #: 163659894
• NCBI GeneID: 16000
• NCBI Accession #: NP_001104745.1
• NCBI GenBank Nucleotide #: NP_001104745.1
• UniProt Accession #: P05017
• Molecular Weight: 7.9 kDa
• NCBI Official Full Name: insulin-like growth factor I isoform 4 preproprotein
• NCBI Official Synonym Full Names: insulin-like growth factor 1
• NCBI Official Symbol: Igf1
• NCBI Official Synonym Symbols: Igf-1; Igf-I; C730016P09Rik
• NCBI Protein Information: insulin-like growth factor I
• UniProt Protein Name: Insulin-like growth factor I
• Protein Family: Insulin-like growth factor
• UniProt Gene Name: Igf1
• UniProt Synonym Gene Names: Igf-1; IGF-I
• UniProt Entry Name: IGF1_MOUSE

NCBI Description

This gene encodes a member of the insulin-like growth factor (IGF) family of proteins that promote growth and development during fetal and postnatal life. This gene is predominantly expressed in the liver and the encoded protein undergoes proteolytic processing to generate a disulfide-linked mature polypeptide. Transgenic disruption of this gene in mice results in reduced postnatal survival and severe growth retardation. Mice lacking the encoded protein exhibit generalized organ hypoplasia including underdevelopment of the central nervous system and developmental defects in bone, muscle and reproductive systems. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]

Uniprot Description

IGF1: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency). IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. Belongs to the insulin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Cellular Component: extracellular space; cell soma; extracellular region; intracellular

Molecular Function: integrin binding; protein serine/threonine kinase activator activity; insulin-like growth factor receptor binding; protein binding; growth factor activity; hormone activity; insulin receptor binding; steroid binding

Biological Process: positive regulation of transcription, DNA-dependent; chondroitin sulfate proteoglycan biosynthetic process; regulation of protein metabolic process; exocrine pancreas development; water homeostasis; positive regulation of glucose import; positive regulation of fibroblast proliferation; proteoglycan biosynthetic process; inner ear development; positive regulation of DNA binding; muscle hypertrophy; nervous system development; positive regulation of mitosis; positive regulation of protein import into nucleus, translocation; regulation of establishment and/or maintenance of cell polarity; regulation of calcium ion transport; positive regulation of cell growth; positive regulation of phosphoinositide 3-kinase cascade; cell activation; positive regulation of peptidyl-tyrosine phosphorylation; branching morphogenesis of a tube; insulin-like growth factor receptor signaling pathway; regulation of gene expression; response to heat; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; alveolus development; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of smooth muscle cell proliferation; myoblast proliferation; positive regulation of glycogen biosynthetic process; negative regulation of caspase activity; positive regulation of activated T cell proliferation; positive regulation of smooth muscle cell migration; detection of mechanical stimulus involved in sensory perception; regulation of translation; negative regulation of cell proliferation; glial cell differentiation; positive regulation of MAPKKK cascade; mammary gland development; positive regulation of cell proliferation; regulation of steroid hormone receptor signaling pathway; positive regulation of granule cell precursor proliferation; regulation of protein amino acid phosphorylation; phosphoinositide-mediated signaling; multicellular organism growth; myotube cell development; satellite cell compartment self-renewal involved in skeletal muscle regeneration; memory; regulation of cell proliferation; myoblast differentiation; cell proliferation; positive regulation of osteoblast differentiation; positive regulation of protein kinase B signaling cascade; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of glycolysis; blood vessel remodeling; positive regulation of Ras protein signal transduction; cell development; positive regulation of DNA replication; lung development