IGF1 active protein

Human IGF1 Protein

Cat. Num. AAA9718686

• Gene Names: IGF1; IGF; MGF; IGFI; IGF-I
• Purity: >95% as determined by SDS-PAGE
• Synonyms: IGF1; Human IGF1 Protein; Recombinant Human IGF1 Protein (Insulin-like growth factor I) expressed in Yeast; IGF-1; IGF-I; IGF1A; IGFI; IGF?I; MGF; Insulin-like growth factor I; IGF1 active protein

• Host: Yeast
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4.
• Sequence: Amino acid sequence derived from human IGF1 (NP_001104754.1) (Gly49-Ala118) was expressed.
• Sequence Length: 137

• Species: Human
• Protein Length: The recombinant human IGF1 consists 70 amino acids and predicts a molecular mass of 7.7 kDa.
• Activity: Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is 15-60 ng/mL.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IGF1 protein in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human IGF1 protein.)

Related Product Information for IGF1 active protein

IGF I, also known as mechano growth factor, somatomedin-C, IGF-I and IGF1, is a secreted protein which belongs to the?insulin family. The insulin family, comprised of insulin, relaxin, insulin-like growth factors I and II (IGF-I and IGF-II) and possibly the beta-subunit of 7S nerve growth factor, represents a group of structurally related polypeptides whose biological functions have diverged. The IGFs, or somatomedins, constitute a class of polypeptides that have a key role in pre-adolescent mammalian growth. IGF-I expression is regulated by GH and mediates postnatal growth, while IGF-II appears to be induced by placental lactogen during prenatal development. IGF1/ IGF-I may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. IGF1/ IGF-I stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1/ IGF-I are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.

NCBI and Uniprot Product Information

• NCBI GI #: 163659901
• NCBI GeneID: 3479
• NCBI Accession #: NP_001104754.1
• NCBI GenBank Nucleotide #: NP_001104754.1
• UniProt Accession #: P05019
• Molecular Weight: 7.7 kDa
• NCBI Official Full Name: insulin-like growth factor I isoform 2
• NCBI Official Synonym Full Names: insulin like growth factor 1
• NCBI Official Symbol: IGF1
• NCBI Official Synonym Symbols: IGF; MGF; IGFI; IGF-I
• NCBI Protein Information: insulin-like growth factor I
• Protein Family: Insulin-like growth factor

NCBI Description

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]