Coagulation Factor II Active Protein | F2 active protein

Coagulation Factor II Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122696

• Gene Names: F2; Cf2; FII; Cf-2; thrombin
• Purity: >96% as determined by SDS-PAGE
• Synonyms: Coagulation Factor II; Coagulation Factor II Protein; Mouse; Recombinant (His Tag); Mouse Coagulation Factor II/FII/F2 Protein (His Tag); Cf-2 Protein; Cf2 Protein; FII Protein; coagulation factor II (thrombin); F2 active protein

• Host: HEK293 Cells
• Purity/Purification: >96% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly618

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 25
• Tag: C-His
• Protein Construction: A DNA sequence encoding the full length of mouse F2 (NP_034298.1)(Met 1-Gly 618) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >2000pmoles/min/ug. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for F2 active protein

Background: Coagulation Factor II Protein (FII, F2 Protein or Prothrombin) is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. Coagulation Factor II Protein (FII, F2 Protein) also plays a role in maintaining vascular integrity during development and postnatal life. Prothrombin/Coagulation Factor II is activated on the surface of a phospholipid membrane that binds the amino end of prothrombin/Coagulation Factor II and factor Va and Xa in Ca-dependent interactions; factor Xa removes the activation peptide and cleaves the remaining part into light and heavy chains. The activation process starts slowly because factor V itself has to be activated by the initial, small amounts of thrombin. Prothrombin/Coagulation Factor II is expressed by the liver and secreted in plasma. Defects in prothrombin/Coagulation Factor II are the cause of factor II deficiency (FA2D). It is very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels. Defects in Coagulation Factor II are also a cause of susceptibility to thrombosis. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation.

Product Categories/Family for F2 active protein

Serine Proteases and Regulators

References

Danneberg J, et al. (1998) Reliable genotyping of the G-20210-A mutation of Coagulation Factor II (prothrombin). Clin Chem. 44(2): 349-51.Redondo M, et al. (1999) Coagulation Factor s II, V, VII, and X, prothrombin gene 20210GA transition, and factor V Leiden in coronary artery disease: high factor V clotting activity is an independent risk factor for myocardial infarction. Arterioscler Thromb Vasc Biol. 19(4): 1020-5.Miletich JP, et al. (1980) The synthesis of sulfated dextran beads for isolation of human plasma Coagulation Factor s II, IX, and X. Anal Biochem. 105(2): 304-10.

NCBI and Uniprot Product Information

• NCBI GI #: 6753798
• NCBI GeneID: 14061
• NCBI Accession #: NP_034298.1
• NCBI GenBank Nucleotide #: NM_010168.2
• UniProt Accession #: P19221
• Molecular Weight: 160,000
• NCBI Official Full Name: prothrombin
• NCBI Official Synonym Full Names: coagulation factor II
• NCBI Official Symbol: F2
• NCBI Official Synonym Symbols: Cf2; FII; Cf-2; thrombin
• NCBI Protein Information: prothrombin
• UniProt Protein Name: Prothrombin
• Protein Family: Prothrombin
• UniProt Gene Name: F2
• UniProt Synonym Gene Names: Cf2
• UniProt Entry Name: THRB_MOUSE

NCBI Description

This gene encodes a vitamin K-dependent glycoprotein coagulation factor that plays an important role in the process of blood coagulation and hemostasis. The encoded protein is an inactive zymogen that undergoes enzymatic cleavage by the coagulation factor Xa to form an active serine protease that converts soluble fibrinogen to insoluble fibrin clot. Most of the mice lacking the encoded protein die at an embryonic stage due to defects in yolk sac vasculature, while the rare nenonates succumb to hemorrhage on the first postnatal day. [provided by RefSeq, Apr 2015]

Uniprot Description

prothrombin: Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Defects in F2 are the cause of factor II deficiency (FA2D). It is a very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels. Genetic variations in F2 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in F2 are the cause of thrombophilia due to thrombin defect (THPH1). It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation. A common genetic variation in the 3-prime untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increased risk of venous thrombosis. Defects in F2 are associated with susceptibility to pregnancy loss, recurrent, type 2 (RPRGL2). A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Belongs to the peptidase S1 family.

Protein type: Protease; Apoptosis; Secreted; EC 3.4.21.5; Secreted, signal peptide

Cellular Component: extracellular matrix; extracellular space; cell; extracellular region; cytosol

Molecular Function: peptidase activity; protein binding; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; calcium ion binding; catalytic activity; receptor binding

Biological Process: negative regulation of proteolysis; platelet activation; positive regulation of blood coagulation; cytosolic calcium ion homeostasis; positive regulation of collagen biosynthetic process; proteolysis; positive regulation of cell growth; positive regulation of phosphoinositide 3-kinase cascade; regulation of cell shape; fibrinolysis; cell surface receptor linked signal transduction; regulation of gene expression; hemostasis; negative regulation of astrocyte differentiation; positive regulation of cell proliferation; response to wounding; acute-phase response; positive regulation of protein amino acid phosphorylation; positive regulation of release of sequestered calcium ion into cytosol; blood coagulation