XCL2 recombinant protein

XCL2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121440

• Gene Names: XCL2; SCM1B; SCYC2; SCM-1b
• Purity: >90% as determined by SDS-PAGE
• Synonyms: XCL2; XCL2 Protein; Human; Recombinant (His Tag); Human XCL2 Protein (His Tag); hCG_1729916 Protein; SCM-1b Protein; SCM1B Protein; SCYC2 Protein; chemokine (C motif) ligand 2; XCL2 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10% gly. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly114

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Val 22
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human XCL2 (Q9UBD3)(Met 1-Gly 114) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for XCL2 recombinant protein

Background: XCL2 is a small cytokine belonging to the XC chemokine family. Chemokines are a group of cytokines. Members of this group of so-called C-Chemokines belong to the SCY family of cytokines and are designated XCL (L for ligand) followed by a number. XCL2 is the new designation of SCM-1-beta. XCL2 is highly related to another chemokine called XCL1. XCL2 gene is located on chromosome 1 in humans. XCL2 is predominantly expressed in activated T cells, but can also be found at low levels in unstimulated cells. XCL2 induces chemotaxis of cells expressing the chemokine receptor XCR1. XCL2 gene has been proposed to participate in pathways (Chemokine signaling pathway, Cytokine-cytokine receptor interaction) and processes (blood circulation, signal transduction, chemotaxis, immune response). XCL2 are expected to have molecular function (chemokine activity) and to localize in various compartments (extracellular space, extracellular region). A putative protein interactor has been described (XCR1). The spliced mRNA putatively encodes a good protein, containing small cytokines (intecrine/chemokine), interleukin-8 like domain; the complete protein appears to be secreted.

References

Kennedy J, et al. (1995) Molecular cloning and functional characterization of human lymphotactin. J Immunol. 155(1):203-9.Yoshida T, et al. (1998) Identification of single C motif-1/lymphotactin receptor XCR. J Biol Chem. 273(26):16551-54.Yoshida T, et al. (1996) Structure and expression of two highly related genes encoding SCM-1/human lymphotactin. FEBS Lett. 395(1):82-8.

NCBI and Uniprot Product Information

• NCBI GI #: 11387207
• NCBI GeneID: 6846
• UniProt Accession #: Q9UBD3
• Molecular Weight: 10319.8
• NCBI Official Full Name: Cytokine SCM-1 beta
• NCBI Official Synonym Full Names: X-C motif chemokine ligand 2
• NCBI Official Symbol: XCL2
• NCBI Official Synonym Symbols: SCM1B; SCYC2; SCM-1b
• NCBI Protein Information: cytokine SCM-1 beta
• UniProt Protein Name: Cytokine SCM-1 beta
• Protein Family: Cytokine
• UniProt Gene Name: XCL2
• UniProt Synonym Gene Names: SCYC2

Uniprot Description

XCL2: Chemotactic activity for lymphocytes but not for monocytes or neutrophils. Belongs to the intercrine gamma family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q24.2

Cellular Component: extracellular region; extracellular space

Molecular Function: CCR chemokine receptor binding; protein binding

Biological Process: blood circulation; G-protein coupled receptor protein signaling pathway; inflammatory response; lymphocyte chemotaxis; monocyte chemotaxis; neutrophil chemotaxis; positive regulation of GTPase activity; positive regulation of inflammatory response; signal transduction