NELL2 recombinant protein

NELL2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121287

• Gene Names: NELL2; NRP2
• Purity: >87% as determined by SDS-PAGE
• Synonyms: NELL2; NELL2 Protein; Human; Recombinant (His Tag); Human NELL2 Protein (His Tag); NRP2 Protein; NEL-like 2 (chicken); NELL2 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >87% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10% gly. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu816

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 22
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human NELL2 (Q99435)(Met 1-Leu 816) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for NELL2 recombinant protein

Background: A brain-enriched secreting signal peptide, NELL2, has been suggested to play multiple roles in the development, survival, and activity of neurons in the mammal. NELL2 is an abundant glycoprotein containing an EGF-like domain in the neural tissues where it has multiple physiological functions by interacting with protein kinase C (PKC). There are two different splicing variant forms of NELL2 identified so far. One is secreted NELL2 (sNELL2) which is a neuron-specific variant and the other is cytosolic NELL2 (cNELL2) which is a non-secreted splicing variant of NELL2. NELL2 is strongly expressed in the brain of adults and fetuses but only weakly in the fetal kidney.

References

Kuroda S, et al. (1999) Biochemical characterization and expression analysis of neural thrombospondin-1-like proteins NELL1 and NELL2. Biochem Biophys Res Commun. 265(1): 79-86.Oyasu M, et al. (2000) Immunocytochemical localization of a neuron-specific thrombospondin-1-like protein, NELL2: light and electron microscopic studies in the rat brain. Brain Res Mol Brain Res. 76(1): 151-60.Nelson BR, et al. (2002) Restricted neural epidermal growth factor-like like 2 (NELL2) expression during muscle and neuronal differentiation. Mech Dev. 119 Suppl 1: 11-9.

NCBI and Uniprot Product Information

• NCBI GI #: 5453766
• NCBI GeneID: 4753
• NCBI Accession #: NP_006150
• NCBI GenBank Nucleotide #: NM_006159.2
• UniProt Accession #: Q99435; Q96JS2; B7Z2U7; B7Z5Q4; B7Z9J5; B7Z9U3
• Molecular Weight: 91,167 Da
• NCBI Official Full Name: protein kinase C-binding protein NELL2 isoform b
• NCBI Official Synonym Full Names: neural EGFL like 2
• NCBI Official Symbol: NELL2
• NCBI Official Synonym Symbols: NRP2
• NCBI Protein Information: protein kinase C-binding protein NELL2
• UniProt Protein Name: Protein kinase C-binding protein NELL2
• Protein Family: Protein kinase C-binding protein
• UniProt Gene Name: NELL2
• UniProt Synonym Gene Names: NRP2

NCBI Description

The protein encoded by this gene is a glycoprotein containing several von Willebrand factor C domains and epidermal growth factor (EGF)-like domains. The encoded protein acts as a homotrimer and is found in the cytoplasm. Several variants encoding a few different isoforms exist, and at least one isoform appears to be a secreted protein. Studies in mouse suggest that this protein plays a role in neural cell growth and differentiation as well as in oncogenesis. [provided by RefSeq, Feb 2009]

Uniprot Description

Required for neuron survival through the modulation of MAPK pathways (). Involved in the regulation of hypothalamic GNRH secretion and the control of puberty ().