Lymphotactin/XCL1 Recombinant Protein | XCL1 recombinant protein

Lymphotactin/XCL1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122907

• Gene Names: Xcl1; LTN; ATAC; Lptn; SCM-1; Scyc1; SCM-1a; AI661682
• Purity: >80% as determined by SDS-PAGE
• Synonyms: Lymphotactin/XCL1; Lymphotactin/XCL1 Protein; Mouse; Recombinant (His Tag); Mouse XCL1 Protein (His Tag); AI661682 Protein; ATAC Protein; Lptn Protein; LTN Protein; SCM-1 Protein; SCM-1a Protein; Scyc1 Protein; chemokine (C motif) ligand 1; XCL1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH7.4, 10% gly. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly114

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Val 22
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse XCL1 (P47993)(Met 1-Gly 114) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for XCL1 recombinant protein

Background: This antimicrobial gene encodes a member of the chemokine superfamily. Chemokines function in inflammatory and immunological responses, inducing leukocyte migration and activation. The encoded protein is a member of the C-chemokine subfamily, retaining only two of four cysteines conserved in other chemokines, and is thought to be specifically chemotactic for T cells. This gene and a closely related family member are located on the long arm of chromosome 1.

References

Nguyen KD, et al. (2008) XCL1 enhances regulatory activities of CD4+ CD25(high) CD127(low/-) T cells in human allergic asthma. J Immunol. 181 (8): 5386-95.
Dong C, et al. (2005) Glycosylated recombinant human XCL1/lymphotactin exhibits enhanced biologic activity. J Immunol Methods. 302 (1-2): 136-44.
Zavala-Flores LM, et al. (2009) Production of biologically active human lymphotactin (XCL1) by Lactococcus lactis. Biotechnol Lett. 31 (2): 215-20.
Guzzo C, Fox JC, Miao H, Volkman BF, Lusso P. Structural Determinants for the Selective Anti-HIV-1 Activity of the All-beta Alternative Conformer of XCL1. Kirchhoff F, ed. Journal of Virology. 2015;89(17):9061-9067.

NCBI and Uniprot Product Information

• NCBI GI #: 1346472
• NCBI GeneID: 16963
• UniProt Accession #: P47993
• NCBI Official Full Name: Lymphotactin
• NCBI Official Synonym Full Names: chemokine (C motif) ligand 1
• NCBI Official Symbol: Xcl1
• NCBI Official Synonym Symbols: LTN; ATAC; Lptn; SCM-1; Scyc1; SCM-1a; AI661682
• NCBI Protein Information: lymphotactin
• UniProt Protein Name: Lymphotactin
• Protein Family: Lymphotactin
• UniProt Gene Name: Xcl1
• UniProt Synonym Gene Names: Lptn; Ltn; Scyc1
• UniProt Entry Name: XCL1_MOUSE

Uniprot Description

XCL1: Chemotactic activity for lymphocytes but not for monocytes or neutrophils. Belongs to the intercrine gamma family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: extracellular space; cell; extracellular region; intracellular; cytosol

Molecular Function: protein homodimerization activity; chemokine activity; chemokine receptor binding; cytokine activity

Biological Process: positive regulation of T cell mediated cytotoxicity; response to virus; negative regulation of transcription factor activity; positive regulation of T cell cytokine production; positive regulation of leukocyte chemotaxis; positive regulation of immune response to tumor cell; negative regulation of T cell cytokine production; chemotaxis; positive regulation of interleukin-10 production; negative regulation of interleukin-2 production; cell-cell signaling; release of sequestered calcium ion into cytosol; negative regulation of interferon-gamma production; regulation of inflammatory response; positive regulation of innate immune response; immune response; positive regulation of release of sequestered calcium ion into cytosol; negative regulation of transcription, DNA-dependent