CD68 recombinant protein

CD68 Protein, Human, Recombinant (aa 1-319, His Tag)

Cat. Num. AAA8120730

• Gene Names: CD68; GP110; LAMP4; SCARD1
• Purity: >98% as determined by SDS-PAGE
• Synonyms: CD68; CD68 Protein; Human; Recombinant (aa 1-319; His Tag); Human CD68/Macrosialin/Gp110 Protein (aa 1-319; GP110 Protein; LAMP4 Protein; SCARD1 Protein; CD68 molecule; CD68 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10% gly. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser319

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Asn 22
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CD68 () was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CD68 recombinant protein

Background: Macrosialin, also known as CD68 and Gp11, is a single-pass type I membrane protein which belongs to theLAMP family. CD68 is highly expressed by blood monocytes and tissue macrophages. It is also expressed in lymphocytes, fibroblasts and endothelial cells. CD68 is expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. CD68 plays a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It is a commonly used marker for macrophages. However, a number of studies have shown that CD68 antibodies react with other hematopoietic and non-hematopoietic cell types, suggesting that CD68 may not be a macrophage-specific antigen. CD68 binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

References

Strobl H. et al., 1995, Br J Haematol. 90 (4): 774-82. Ogawa Y. et al., 1995, Pathol Int. 45 (9): 698-701. Sadovnikova E. et al., 2002,Leukemia. 16 (10): 2019-26. Gottfried E. et al., 2008, Scand J Immunol. 67 (5): 453-63. Strojnik T. et al., 2009, Anticancer Res. 29 (8): 3269-79.

NCBI and Uniprot Product Information

• NCBI GI #: 298665
• NCBI GeneID: 968
• UniProt Accession #: P34810; Q53HR6; Q53XI3; Q96BI7; B4DVT4
• Molecular Weight: 37,408 Da
• NCBI Official Full Name: CD68
• NCBI Official Synonym Full Names: CD68 molecule
• NCBI Official Symbol: CD68
• NCBI Official Synonym Symbols: GP110; LAMP4; SCARD1
• NCBI Protein Information: macrosialin; CD68 antigen; macrophage antigen CD68; scavenger receptor class D, member 1
• UniProt Protein Name: Macrosialin
• Protein Family: Macrosialin
• UniProt Gene Name: CD68
• UniProt Entry Name: CD68_HUMAN

NCBI Description

This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CD68: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. Belongs to the LAMP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: membrane; lysosomal membrane; plasma membrane; integral to membrane; endosome membrane