Western Blot (WB)
(Western blot image of GST-recombinant human VEGFR-1 (89kDa), VEGFR-2 (110kDa), and VEGFR-3 (86kDa) C-terminal regions. The blots were probed with rabbit polyclonal anti-VEGFR-2 (a.a. 1304-1317), anti-VEGFR-2 (Tyr-801), and anti-VEGFR-3 (a.a. 1285-1298).)
VEGFR-3 recombinant protein
VEGFR-3 Recombinant Protein
Applications
Western Blot
Synonyms
VEGFR-3; VEGFR-3 Recombinant Protein; VEGFR-3 recombinant protein
Host
Lysate
Form/Format
VEGFR-3 recombinant protein lysate is supplied at aconcentration of 2ng/ul in electrophoresis sample buffer (62.5mM Tris pH6.8, 2% SDS, 5% glycerol, 0.003% bromophenolblue, 0.9% beta-mercaptoethanol).
Applicable Applications for VEGFR-3 recombinant protein
Western Blot (WB)
Application Notes
WB: 10ul/lane
Preparation and Storage
Store at -20 degree C. Do not boil ordilute. Stable for 1 year.
Western Blot (WB)
(Western blot image of GST-recombinant human VEGFR-1 (89kDa), VEGFR-2 (110kDa), and VEGFR-3 (86kDa) C-terminal regions. The blots were probed with rabbit polyclonal anti-VEGFR-2 (a.a. 1304-1317), anti-VEGFR-2 (Tyr-801), and anti-VEGFR-3 (a.a. 1285-1298).)
Western Blot (WB)
(Western blot image of GST-recombinant human VEGFR-1 (89kDa), VEGFR-2 (110kDa), and VEGFR-3 (86kDa) C-terminal regions. The blots were probed with rabbit polyclonal anti-VEGFR-2 (a.a. 1304-1317), anti-VEGFR-2 (Tyr-801), and anti-VEGFR-3 (a.a. 1285-1298).)
Related Product Information for VEGFR-3 recombinant protein
Vascular endothelial growth factor receptor-2 (VEGFR-2/Flk-1/KDR) is the primary receptor for VEGF in endothelial cells. Other VEGFR family members, VEGFR-1 (Flt-1) and VEGFR-3 (Flt-4), can also transduce the intracellular signals of VEGF. However, the role of VEGFR-1 is observed mainly during embryonic angiogenesis and VEGFR-3 signaling may be restricted to specific types of endothelial cells. Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr-951/996) and in the tyrosine kinase catalytic domain (Tyr-1054/1059). Other sites, Tyr-1175 and Tyr-1212 provide docking sites for downstream signaling molecules. Activation of VEGFR-2 also phosphorylates Tyr-801, leading to PI3-kinase-Akt activation and increases in endothelial nitric oxide synthase activity. Human recombinant VEGFR-3 protein includes the cytoplasmic region from VEGFR-3 and an N-terminal GST fusion protein (86kDa). The protein is detected by rabbit polyclonal anti-VEFGR-3 (a.a. 1285-1298) antibody .
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Product Notes
The VEGFR-3 (Catalog #AAA474637) is a Recombinant Protein produced from Lysate and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's VEGFR-3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 10ul/lane. Researchers should empirically determine the suitability of the VEGFR-3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VEGFR-3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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