Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Immunohistochemistry (IHC) (Anti-ATF7 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.)

Goat ATF7 Polyclonal Antibody | anti-ATF7 antibody

Goat Polyclonal to Human ATF7

Gene Names
ATF7; ATFA
Reactivity
Bat, Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Pig, Rabbit, Rat
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Immunoaffinity Purified
Synonyms
ATF7; Polyclonal Antibody; Goat Polyclonal to Human ATF7; Anti-ATF7 Antibody (C-Terminus) IHC-plus; ATFA; Transcription factor ATF-A; Human ATF7; anti-ATF7 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Bat, Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Pig, Rabbit, Rat
Clonality
Polyclonal
Specificity
Human ATF7. This antibody is expected to recognise all three reported isoforms (NP_001123531.1, NP_006847.1 and NP_001123532.1).
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
483
Applicable Applications for anti-ATF7 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA (1:16000), IHC-P (3.75 ug/ml), WB (0.5 - 2 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Target Species
Human
Immunogen Description
Synthetic peptide C-HVIMTPQSQSAGR from the C-terminus of human ATF7 (NP_001123531.1; NP_006847.1; NP_001123532.1).
Immunogen Type
Synthetic peptide
Immunogen
ATF7 antibody was raised against synthetic peptide C-HVIMTPQSQSAGR from the C-terminus of human ATF7 (NP_001123531.1; NP_006847.1; NP_001123532.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig (100%); Lizard (92%).
Antigen Modification
C-Terminus
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-ATF7 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.)

Immunohistochemistry (IHC) (Anti-ATF7 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.)

Western Blot (WB)

(Antibody staining (0.5 ug/ml) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)

Western Blot (WB) (Antibody staining (0.5 ug/ml) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)
Product Categories/Family for anti-ATF7 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51,757 Da
NCBI Official Full Name
cyclic AMP-dependent transcription factor ATF-7 isoform 2
NCBI Official Synonym Full Names
activating transcription factor 7
NCBI Official Symbol
ATF7
NCBI Official Synonym Symbols
ATFA
NCBI Protein Information
cyclic AMP-dependent transcription factor ATF-7; transcription factor ATF-A
UniProt Protein Name
Cyclic AMP-dependent transcription factor ATF-7
UniProt Gene Name
ATF7
UniProt Synonym Gene Names
ATFA; cAMP-dependent transcription factor ATF-7
UniProt Entry Name
ATF7_HUMAN

Uniprot Description

ATF7: Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]- 3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family. Belongs to the bZIP family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; sequence-specific DNA binding; metal ion binding; mitogen-activated protein kinase binding; transcription factor binding; transcription factor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; viral reproduction; negative regulation of transcription from RNA polymerase II promoter

Research Articles on ATF7

Similar Products

Product Notes

The ATF7 atf7 (Catalog #AAA242188) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human ATF7 reacts with Bat, Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Pig, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATF7 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA (1:16000), IHC-P (3.75 ug/ml), WB (0.5 - 2 ug/ml) Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the ATF7 atf7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATF7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.