Typical Testing Data/Standard Curve
Rat CD265 ELISA Kit | TNFRSF11A elisa kit
Rat CD265 ELISA Kit
Reactivity
Rat
Applications
ELISA
Synonyms
CD265; Rat CD265 ELISA Kit; RANK; Tumor necrosis factor receptor superfamily member 11A; Osteoclast differentiation factor receptor; ODFR; Receptor activator of NF-KB; TNFRSF11A elisa kit
Reactivity
Rat
Specificity
The Rat CD265 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat CD265 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
Sequence Length
633
Applicable Applications for TNFRSF11A elisa kit
Sandwich ELISA (EIA)
Samples
Cell Culture Supernatants, Serum.
Sensitivity
10 pg/ml.
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for TNFRSF11A elisa kit
Principle of the Assay||The Rat CD265 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CD265 in Cell Culture Supernatants, Serum. This assay employs an antibody specific for Rat CD265 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD265 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CD265 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD265 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Receptor Activator of Nuclear Factor kappa B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain (residues 234-616), which contain three putative TRAF-binding domains (termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK. The standard in this kit is recombinant human RANK with the sequence of Q29-G213 aa. It is a dipolymer which compose of two chains, and the molecular weight of each is 48kda.
Background/Introduction: Receptor Activator of Nuclear Factor kappa B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain (residues 234-616), which contain three putative TRAF-binding domains (termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK. The standard in this kit is recombinant human RANK with the sequence of Q29-G213 aa. It is a dipolymer which compose of two chains, and the molecular weight of each is 48kda.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
67,607 Da
UniProt Protein Name
Protein Tnfrsf11a
Protein Family
UniProt Gene Name
Tnfrsf11a
UniProt Entry Name
F1M8Z6_RAT
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Product Notes
The Rat TNFRSF11A tnfrsf11a (Catalog #AAA8244637) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8244637 ELISA Kit recognizes Rat TNFRSF11A. AAA Biotech's CD265 can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (EIA). Researchers should empirically determine the suitability of the TNFRSF11A tnfrsf11a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD265, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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