Western Blot (WB)
(Western blot analysis of human CD265 (soluble) using recombinant human CD265 (soluble) diluted between 1.5 - 3.0ng/lane. Detection was performed using Rabbit anti Human CD265 antibody (MBS226110) followed by a compatible secondary antibody.)
Rabbit anti-Human CD265 Polyclonal Antibody | anti-CD265 antibody
Rabbit anti Human CD265 (soluble):Preservative Free
Purified IgG - lyophilized
WB: 0.1ug/ml to 0.2ug/ml
After reconstitution store at -20 degree C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Western Blot (WB)
(Western blot analysis of human CD265 (soluble) using recombinant human CD265 (soluble) diluted between 1.5 - 3.0ng/lane. Detection was performed using Rabbit anti Human CD265 antibody (MBS226110) followed by a compatible secondary antibody.)
Western Blot (WB)
(Western blot analysis of human CD265 (soluble) using recombinant human CD265 (soluble) diluted between 1.5 - 3.0ng/lane. Detection was performed using Rabbit anti Human CD265 antibody (MBS226110) followed by a compatible secondary antibody.)
CD265 has major functions in bone remodeling, signaling to regulate osteoclast formation, activation and survival in normal bone modeling and remodeling in an array of pathological conditions characterized by upregulated bone turnover (Boyce & Xing 2008). CD265 signaling is induced upon binding of its ligand RANKL which results in the recruitment of one of several adapter proteins including TRAF6. This can result in the activation of the NF-kappaB, MAPK or c-Src signaling pathways (Nagy & Penninger 2015).
Beyond its crucial function in osteoclastogenesis, CD265 has also been implicated in other physiological processes including immunotolerance, organogenesis of the immune system and osteoimmunology (Sigl et al. 2016).
NCBI and Uniprot Product Information
Uniprot Description
TNFRSF11A: Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. Binds to the clefts between the subunits of the TNFSF11 ligand trimer to form a heterohexamer. Interacts with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6. Interacts (via cytoplasmic domain) with GAB2. Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 18q22.1
Cellular Component: external side of plasma membrane; integral to plasma membrane; plasma membrane
Molecular Function: cytokine binding; protein binding; receptor activity; transmembrane receptor activity; tumor necrosis factor receptor activity
Biological Process: activation of NF-kappaB transcription factor; adaptive immune response; cell-cell signaling; circadian thermoregulation; inflammatory response; osteoclast differentiation; positive regulation of cell proliferation; positive regulation of JNK activity; positive regulation of transcription factor activity; regulation of apoptosis; response to cytokine stimulus; response to lipopolysaccharide; signal transduction; tumor necrosis factor-mediated signaling pathway
Disease: Familial Expansile Osteolysis; Osteopetrosis, Autosomal Recessive 7; Paget Disease Of Bone
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Product Notes
The CD265 tnfrsf11a (Catalog #AAA226110) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Human CD265 (soluble):Preservative Free reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD265 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: 0.5ug/ml to 2.0ug/ml WB: 0.1ug/ml to 0.2ug/ml. Researchers should empirically determine the suitability of the CD265 tnfrsf11a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD265, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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