Rabbit anti-Human ACVR2B Polyclonal Antibody | anti-ACVR2B antibody

ACVR2B Antibody (N-term)

Cat. Num. AAA9209648

• Gene Names: ACVR2B; HTX4; ACTRIIB; ActR-IIB
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: ACVR2B; Polyclonal Antibody; ACVR2B Antibody (N-term); Activin receptor type-2B; Activin receptor type IIB; ACTR-IIB; anti-ACVR2B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This ACVR2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected between aa 1 and 30 of human ACVR2B.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 1-30
• Sequence Length: –

• Applicable Applications for anti-ACVR2B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Cellular Location: Cell membrane; Single-pass type I membraneprotein
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysate from human pancreas tissue lysate, using ACVR2B Antibody (S14). MBS9209648 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.)

Western Blot (WB)

(Western blot analysis of ACVR2B (N-term) in Jurkat cell line lysates (35ug/lane).ACVR2B (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of anti-ACVR2B Antibody (N-term) in mouse heart, kidney and brain lysates (35ug/lane). ACVR2B (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-ACVR2B antibody

Transmembrane serine/threonine kinase activin type-2 receptor forming an activin receptor complex with activin type-1serine/threonine kinase receptors (ACVR1,ACVR1B or ACVR1c). Transduces the activin signal from the cell surface to the cytoplasmand is thus regulating many physiological andpathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine orautocrine role in follicular development in the ovary. Within the receptor complex, thetype-2 receptors act as a primary activin receptors (binds activin-A/INHBA,activin-B/INHBB as well as inhibin-A/INHA-INHBA). The type-1 receptors like ACVR1B act as downstream transducers ofactivin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine-threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C-terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into thenucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which isrecruited to ACVR1B through FKBP1A, canprevent the association of SMAD2 and SMAD3 with the activin receptor complex, there by blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor.

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal througha heteromeric complex of receptor serine kinaseswhich include at least two type I (I and IB) andtwo type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of aligand-binding extracellular domain with cysteine-rich region, a transmembrane domain,and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors areessential for signaling; and type II receptors arerequired for binding ligands and for expression oftype I receptors. Type I and II receptors form astable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptors are considered to be constitutively active kinases. ACVR2B (activin A type IIB receptor) displays a 3- to 4-fold highe raffinity for the ligand than activin A type IIreceptor.

Product Categories/Family for anti-ACVR2B antibody

Signal Transduction

References

Harrison, C.A., et al., J. Biol. Chem. 279(27):28036-28044 (2004). Martins da Silva, S.J., et al., Dev. Biol. 266(2):334-345 (2004). Casagrandi, D., et al., Mol. Hum. Reprod. 9(4):199-203 (2003). Shin, B.K., et al., J. Biol. Chem. 278(9):7607-7616 (2003). Schneider-Kolsky, M.E., et al., Placenta 23(4):294-302 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 116734708
• NCBI GeneID: 93
• NCBI Accession #: NP_001097.2
• NCBI GenBank Nucleotide #: NM_001106.3
• UniProt Accession #: Q13705; Q4VAV0
• NCBI Official Full Name: activin receptor type-2B
• NCBI Official Synonym Full Names: activin A receptor, type IIB
• NCBI Official Symbol: ACVR2B
• NCBI Official Synonym Symbols: HTX4; ACTRIIB; ActR-IIB
• NCBI Protein Information: activin receptor type-2B
• UniProt Protein Name: Activin receptor type-2B
• Protein Family: Activin receptor
• UniProt Gene Name: ACVR2B
• UniProt Synonym Gene Names: ACTR-IIB
• UniProt Entry Name: AVR2B_HUMAN

NCBI Description

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptors are considered to be constitutively active kinases. This gene encodes activin A type IIB receptor, which displays a 3- to 4-fold higher affinity for the ligand than activin A type II receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

ACVR2B: a tyrosine-kinase like receptor kinase of the STKR family. The holoreceptor receptor is a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. Each is composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic kinase domain. Type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptor kinases are apparently constitutively active. The IIB receptor displays a 3- to 4-fold higher affinity for activin A than the II receptor.

Protein type: Protein kinase, TKL; Protein kinase, Ser/Thr (receptor); Membrane protein, integral; EC 2.7.11.30; Kinase, protein; TKL group; STKR family; Type2 subfamily

Chromosomal Location of Human Ortholog: 3p22

Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; receptor complex

Molecular Function: transforming growth factor beta receptor activity; protein serine/threonine kinase activity; protein binding; metal ion binding; growth factor binding; protein serine/threonine/tyrosine kinase activity; activin binding; activin receptor activity, type II; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: heart development; activin receptor signaling pathway; palate development; signal transduction; protein amino acid phosphorylation; post-embryonic development; BMP signaling pathway; anterior/posterior pattern formation; positive regulation of activin receptor signaling pathway; regulation of transcription, DNA-dependent; transmembrane receptor protein serine/threonine kinase signaling pathway; embryonic foregut morphogenesis; lymphangiogenesis; pancreas development; response to glucose stimulus; mesoderm development; kidney development; gastrulation with mouth forming second; positive regulation of bone mineralization; odontogenesis of dentine-containing teeth; positive regulation of osteoblast differentiation; organ growth; skeletal morphogenesis; insulin secretion; activation of protein kinase activity; blood vessel remodeling; determination of left/right symmetry; lung development

Disease: Heterotaxy, Visceral, 4, Autosomal