Ephrin-A1 ELISA Pair Set | EFNA1 elisa pair set

Ephrin-A1 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118316

• Synonyms: Ephrin-A1; Ephrin-A1 Matched ELISA Antibody Pair Set; Human; Human Ephrin-A1/EFNA1 ELISA Pair Set; ephrin-A1; B61 Matched ELISA Antibody Pair Set; ECKLG Matched ELISA Antibody Pair Set; EFL1 Matched ELISA Antibody Pair Set; EFNA1 Matched ELISA Antibody Pair Set; EPLG1 Matched ELISA Antibody Pair Set; LERK-1 Matched ELISA Antibody Pair Set; LERK1 Matched ELISA Antibody Pair Set; TNFAIP4 Matched ELISA Antibody Pair Set; EFNA1 elisa pair set

• Specificity: Quantitative determination of Human Ephrin-A1

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 39.06-2500pg/mL
• Sensitivity: 39pg/ml
• Materials Provided: 1. Capture Antibody 0.5 mg/mL of mouse anti-EFNA1 monoclonal antibody, Dilute to a working concentration of 2ug/mL in CBS before coating. ; 2. Detection Antibody 1 mg/mL rabbit anti-EFNA1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.2ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 140 ng of recombinant EFNA1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2500 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for EFNA1 elisa pair set

EPH-related receptor tyrosine kinase ligand 1 (abbreviated as Ephrin-A1) also known as ligand of eph-related kinase 1 or EFNA1, is a member of the ephrin (EPH) family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin-A1/EFNA1 and its Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. Ephrin-A1 and one of its receptor EphA2 were expressed in xenograft endothelial cells and also tumor cells and play a role in human cancers, at least in part by influencing tumor neovascularization.

This Ephrin-A1 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Ephrin-A1. It contains Human Ephrin-A1 capture antibody, Human Ephrin-A1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Ephrin-A1 protein.

References

Deroanne C, et al. (2003) EphrinA1 inactivates integrin-mediated vascular smooth muscle cell spreading via the Rac/PAK pathway. J Cell Sci. 116(7): 1367-76.Ojima T, et al. (2006) EphrinA1 inhibits vascular endothelial growth factor-induced intracellular signaling and suppresses retinal neovascularization and blood-retinal barrier breakdown. Am J Pathol. 168(1): 331-9.Wu D, et al. (2004) Prognostic value of EphA2 and EphrinA-1 in squamous cell cervical carcinoma. Gynecol Oncol. 94(2): 312-9.