Rabbit Tie-1 Polyclonal Antibody | anti-Tie1 antibody

Anti-Tie-1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103432

• Gene Names: Tie1; TIE; tie-1; D430008P04Rik
• Reactivity: Mouse; Predicted to React with: Human, Zebrafish (Species predicted to react based on 100% sequence homology)
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: Tie-1; Polyclonal Antibody; Anti-Tie-1 Antibody; Rabbit Polyclonal; Tie-1 Antibody; Rabbit PAb; Antigen Affinity Purified; tyrosine kinase with immunoglobulin-like and EGF-like domains 1; Anti-D430008P04Rik Antibody; Anti-TIE Antibody; Anti-tie-1 Antibody; anti-Tie1 antibody

• Host: Rabbit
• Reactivity: Mouse; Predicted to React with: Human, Zebrafish (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse Tie-1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-Tie1 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:200-1:1000
• Immunogen: A synthetic peptide corresponding to the center region of the Mouse Tie-1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse Tie-1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse TIE1 in mouse stomach with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse TIE1 in mouse kidney with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-Tie1 antibody

Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 also known as Tie1 is an angiopoietin receptor and is an orphan receptor tyrosine kinase that is expressed almost exclusively in endothelial cells and that is required for normal embryonic vascular development. The receptor tyrosine kinase Tie1 is expressed primarily in vascular endothelial cells. The receptor has also been detected in epithelial tumours in breast, thyroid and gastric cancers and in tumour cell lines where it appears as a 45 kDa truncated receptor fragment. Tie1 promotes endothelial cell survival, but other studies have suggested that the Tie1 kinase has little to no activity. Embryos deficient in Tie1 failed to establish structural integrity of vascular endothelial cells, resulting in oedema and subsequently localized haemorrhage. Tie1 is significantly higher in human aortic endothelial cells than in human umbilical vein endothelial cells. Additionally, attachment of cells of monocytic lineage to endothelial cells is also enhanced by Tie1 expression. Collectively Tie1 has a proinflammatory property and may play a role in endothelial inflammatory diseases such as atherosclerosis.

Product Categories/Family for anti-Tie1 antibody

Receptor Tyrosine Kinases (RTKs)

References

Chan B, et al. (2008) Receptor tyrosine kinase Tie-1 overexpression in endothelial cells upregulates adhesion molecules. Biochem Biophys Res Commun. 371(3): 475-9.Sato TN, et al. (1995) Distinct roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation. Nature. 376(6535): 70-4.Rees KA, et al. (2007) The receptor tyrosine kinase Tie1 is expressed and activated in epithelial tumour cell lines. Int J Oncol. 31(4): 893-7.Kontos CD, et al. (2002) The endothelial receptor tyrosine kinase Tie1 activates phosphatidylinositol 3-kinase and Akt to inhibit apoptosis. Mol Cell Biol. 22(6): 1704-13.

NCBI and Uniprot Product Information

• NCBI GI #: 144446133
• NCBI GeneID: 21846
• NCBI Accession #: NP_035717.2
• NCBI GenBank Nucleotide #: NM_011587.2
• UniProt Accession #: Q06806; Q811F4; Q8BGI2
• Molecular Weight: 124,583 Da
• NCBI Official Full Name: tyrosine-protein kinase receptor Tie-1
• NCBI Official Synonym Full Names: tyrosine kinase with immunoglobulin-like and EGF-like domains 1
• NCBI Official Symbol: Tie1
• NCBI Official Synonym Symbols: TIE; tie-1; D430008P04Rik
• NCBI Protein Information: tyrosine-protein kinase receptor Tie-1; tyrosine kinase receptor 1
• UniProt Protein Name: Tyrosine-protein kinase receptor Tie-1
• Protein Family: Tyrosine-protein kinase receptor
• UniProt Gene Name: Tie1
• UniProt Synonym Gene Names: Tie; Tie-1
• UniProt Entry Name: TIE1_MOUSE

Uniprot Description

TIE1: Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis. Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.

Protein type: Membrane protein, integral; Kinase, protein; Protein kinase, tyrosine (receptor); Protein kinase, TK; EC 2.7.10.1; TK group; Tie family

Cellular Component: membrane; integral to membrane; plasma membrane

Molecular Function: transferase activity; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: blood vessel development; negative regulation of angiogenesis; response to retinoic acid; in utero embryonic development; angiogenesis; vasculogenesis; negative regulation of cell migration; protein amino acid phosphorylation; phosphorylation; plasma membrane fusion