NUDT15 sirna

NUDT15 siRNA (Mouse)

Cat. Num. AAA8228104

• Gene Names: Nudt15; MTH2; 6530403O17; A730068G11Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NUDT15; NUDT15 siRNA (Mouse); MTH2; Probable 8-oxo-dGTP diphosphatase NUDT15; 8-oxo-dGTPase NUDT15; 7.8-dihydro-8-oxoguanine-triphosphatase NUDT15; MutT homolog 2; mMTH2; Nucleoside diphosphate-linked moiety X motif 15; Nudix motif 15; NUDT15 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: NUDT15 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 170

• Applicable Applications for NUDT15 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NUDT15 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NUDT15 sirna

siRNA to inhibit NUDT15 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 27369730
• NCBI GeneID: 214254
• NCBI Accession #: NP_766115.1
• NCBI GenBank Nucleotide #: NM_172527.2
• UniProt Accession #: Q8BG93
• Molecular Weight: 19,576 Da
• NCBI Official Full Name: probable 8-oxo-dGTP diphosphatase NUDT15
• NCBI Official Synonym Full Names: nudix (nucleoside diphosphate linked moiety X)-type motif 15
• NCBI Official Symbol: Nudt15
• NCBI Official Synonym Symbols: MTH2; 6530403O17; A730068G11Rik
• NCBI Protein Information: probable 8-oxo-dGTP diphosphatase NUDT15
• UniProt Protein Name: Probable 8-oxo-dGTP diphosphatase NUDT15
• Protein Family: Nudix hydrolase
• UniProt Gene Name: Nudt15
• UniProt Synonym Gene Names: Mth2; 8-oxo-dGTPase NUDT15; mMTH2; Nudix motif 15
• UniProt Entry Name: NUD15_MOUSE

Uniprot Description

NUDT15: Mediates the hydrolysis of some nucleoside diphosphate derivatives. Can degrade 8-oxo-dGTP in vitro, suggesting that it may remove an oxidatively damaged form of guanine (7,8-dihydro-8- oxoguanine) from DNA and the nucleotide pool, thereby preventing misincorporation of 8-oxo-dGTP into DNA thus preventing A:T to C:G transversions. Its substrate specificity in vivo however remains unclear. May have a role in DNA synthesis and cell cycle progression through the interaction with PCNA. Belongs to the Nudix hydrolase family.

Protein type: Hydrolase; EC 3.6.1.55

Molecular Function: hydrolase activity; metal ion binding; 8-oxo-7,8-dihydroguanine triphosphatase activity

Biological Process: response to reactive oxygen species; dGTP catabolic process; purine deoxyribonucleoside triphosphate catabolic process