Western Blot (WB)
(Western blot analysis of NUDT15 expression in HT29 (A), SW480 (B) whole cell lysates.)
Rabbit anti-Human NUDT15 Polyclonal Antibody | anti-NUDT15 antibody
Anti-NUDT15 Antibody
Western Blot (WB)
(Western blot analysis of NUDT15 expression in HT29 (A), SW480 (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of NUDT15 expression in HT29 (A), SW480 (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of NUDT15 staining in human stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of NUDT15 staining in human stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an enzyme that belongs to the Nudix hydrolase superfamily. Members of this superfamily catalyze the hydrolysis of nucleoside diphosphates, including substrates like 8-oxo-dGTP, which are a result of oxidative damage, and can induce base mispairing during DNA replication, causing transversions. The encoded enzyme is a negative regulator of thiopurine activation and toxicity. Mutations in this gene result in poor metabolism of thiopurines, and are associated with thiopurine-induced early leukopenia. Multiple pseudogenes of this gene have been identified. [provided by RefSeq, Apr 2016]
Uniprot Description
NUDT15: Mediates the hydrolysis of some nucleoside diphosphate derivatives. Can degrade 8-oxo-dGTP in vitro, suggesting that it may remove an oxidatively damaged form of guanine (7,8-dihydro-8- oxoguanine) from DNA and the nucleotide pool, thereby preventing misincorporation of 8-oxo-dGTP into DNA thus preventing A:T to C:G transversions. Its substrate specificity in vivo however remains unclear. May have a role in DNA synthesis and cell cycle progression through the interaction with PCNA. Belongs to the Nudix hydrolase family.
Protein type: Hydrolase; EC 3.6.1.55
Chromosomal Location of Human Ortholog: 13q14.2
Cellular Component: cytosol
Molecular Function: 8-oxo-7,8-dihydroguanine triphosphatase activity; metal ion binding; nucleoside-diphosphatase activity
Biological Process: dGTP catabolic process
Research Articles on NUDT15
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Product Notes
The NUDT15 nudt15 (Catalog #AAA8245227) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NUDT15 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NUDT15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1/500 - 1/2000; IHC: 1/50 - 1/100. Researchers should empirically determine the suitability of the NUDT15 nudt15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUDT15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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