GREM1 sirna

GREM1 siRNA (Mouse)

Cat. Num. AAA8230415

• Gene Names: Grem1; ld; Drm; Grem; Cktsf1b1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GREM1; GREM1 siRNA (Mouse); CKTSF1B1; DRM; Gremlin-1; Cysteine knot superfamily 1. BMP antagonist 1; Down-regulated in Mos-transformed cells protein; GREM1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: GREM1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 184

• Applicable Applications for GREM1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GREM1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GREM1 sirna

siRNA to inhibit GREM1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6754068
• NCBI GeneID: 23892
• NCBI Accession #: NP_035954.1
• NCBI GenBank Nucleotide #: NM_011824.4
• UniProt Accession #: O70326
• Molecular Weight: 20,710 Da
• NCBI Official Full Name: gremlin-1
• NCBI Official Synonym Full Names: gremlin 1
• NCBI Official Symbol: Grem1
• NCBI Official Synonym Symbols: ld; Drm; Grem; Cktsf1b1
• NCBI Protein Information: gremlin-1
• UniProt Protein Name: Gremlin-1
• Protein Family: Gremlin
• UniProt Gene Name: Grem1
• UniProt Synonym Gene Names: Cktsf1b1; Drm
• UniProt Entry Name: GREM1_MOUSE

Uniprot Description

GREM1: Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Acts as inhibitor of monocyte chemotaxis. Interacts with SLIT1 and SLIT2 in a glycosylation- dependent manner. By high glucose through TGFB1-mediated pathways in mesangial cell. Down-regulated in tumor cell lines. Highly expressed in small intestine, fetal brain and colon. Weakly expressed in brain, ovary, prostate, pancreas and skeletal muscle. In brain found in the region localized around the internal capsule in the large subcortical nuclei, including caudate, putamen, substantia nigra, thalamus and subthalamus. Predominantly expressed in normal cells including neurons, astrocytes and fibroblasts. Belongs to the DAN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: heparan sulfate proteoglycan binding; transmembrane receptor protein tyrosine kinase activator activity; vascular endothelial growth factor receptor 2 binding; cytokine activity; receptor agonist activity

Biological Process: limb development; transmembrane receptor protein tyrosine kinase activation (dimerization); collagen fibril organization; positive regulation of transcription, DNA-dependent; negative regulation of chondrocyte differentiation; positive regulation of receptor internalization; cell morphogenesis; positive regulation of NF-kappaB import into nucleus; signal transduction; negative regulation of BMP signaling pathway; activation of NF-kappaB transcription factor; negative regulation of bone mineralization; cell-cell signaling; positive regulation of cell proliferation; negative regulation of leukocyte chemotaxis; angiogenesis; proximal/distal pattern formation; embryonic limb morphogenesis; determination of dorsal identity; positive regulation of telomerase activity; regulation of stress-activated MAPK cascade; organ morphogenesis; positive regulation of angiogenesis; cell migration during sprouting angiogenesis; ureteric bud branching; regulation of focal adhesion formation; negative regulation of bone remodeling; endothelial cell migration; negative regulation of osteoblast proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis