Rat GremLin 1 ELISA Kit | GREM1 elisa kit

Rat GremLin 1 ELISA Kit

Cat. Num. AAA761770

• Gene Names: Grem1; ld; Drm; Grem; Cktsf1b1
• Reactivity: Rat
• Synonyms: GremLin 1; Rat GremLin 1 ELISA Kit; GREM1 (GremLin 1)/DAND2/DRM/Cell proliferation-inducing gene 2 protein/CKTSF1B1/gremLin 1; cysteine knot superfamily; homolog/DAN domain family member 2/GREMLIN/Down-regulated in Mos-transformed cells protein/DRMM/gremLin/gremLin-1/gremLin 1-like protein/IHG-2/Increased in high glucose protein 2/increased in high glucose-2/proliferation-inducing gene 2; GREM1 elisa kit

• Reactivity: Rat
• Sequence Length: 184

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for GREM1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat GREM1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat GREM1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat GREM1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat GREM1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 15929743
• NCBI GeneID: 23892
• Molecular Weight: 20,710 Da
• NCBI Official Full Name: Gremlin 1
• NCBI Official Synonym Full Names: gremlin 1, DAN family BMP antagonist
• NCBI Official Symbol: Grem1
• NCBI Official Synonym Symbols: ld; Drm; Grem; Cktsf1b1
• NCBI Protein Information: gremlin-1
• UniProt Protein Name: Gremlin-1
• Protein Family: Gremlin
• UniProt Gene Name: Grem1
• UniProt Synonym Gene Names: Cktsf1b1; Drm
• UniProt Entry Name: GREM1_MOUSE

Uniprot Description

GREM1: Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Acts as inhibitor of monocyte chemotaxis. Interacts with SLIT1 and SLIT2 in a glycosylation- dependent manner. By high glucose through TGFB1-mediated pathways in mesangial cell. Down-regulated in tumor cell lines. Highly expressed in small intestine, fetal brain and colon. Weakly expressed in brain, ovary, prostate, pancreas and skeletal muscle. In brain found in the region localized around the internal capsule in the large subcortical nuclei, including caudate, putamen, substantia nigra, thalamus and subthalamus. Predominantly expressed in normal cells including neurons, astrocytes and fibroblasts. Belongs to the DAN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: cell surface; extracellular region; extracellular space

Molecular Function: cytokine activity; heparan sulfate proteoglycan binding; receptor agonist activity; transmembrane receptor protein tyrosine kinase activator activity; vascular endothelial growth factor receptor 2 binding

Biological Process: activation of NF-kappaB transcription factor; angiogenesis; cell migration during sprouting angiogenesis; cell morphogenesis; cell-cell signaling; collagen fibril organization; determination of dorsal identity; embryonic limb morphogenesis; endothelial cell migration; limb development; negative regulation of apoptosis; negative regulation of BMP signaling pathway; negative regulation of bone mineralization; negative regulation of bone remodeling; negative regulation of cell growth; negative regulation of chondrocyte differentiation; negative regulation of leukocyte chemotaxis; negative regulation of osteoblast proliferation; negative regulation of transcription, DNA-dependent; organ morphogenesis; positive regulation of angiogenesis; positive regulation of cell proliferation; positive regulation of NF-kappaB import into nucleus; positive regulation of receptor internalization; positive regulation of telomerase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; proximal/distal pattern formation; regulation of focal adhesion formation; regulation of stress-activated MAPK cascade; signal transduction; ureteric bud branching