Rabbit anti-Mouse Gremlin 1/GREM1 Polyclonal Antibody | anti-GREM1 antibody

Anti-Gremlin 1/GREM1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8110778

• Gene Names: Grem1; ld; Drm; Grem; Cktsf1b1
• Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: Gremlin 1/GREM1; Polyclonal Antibody; Anti-Gremlin 1/GREM1 Antibody; Rabbit Polyclonal; Gremlin 1/GREM1 Antibody; Rabbit PAb; Antigen Affinity Purified; gremlin 1; DAN family BMP antagonist; Anti-Cktsf1b1 Antibody; Anti-Drm Antibody; Anti-Grem Antibody; Anti-ld Antibody; anti-GREM1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse Gremlin 1/GREM1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-GREM1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:100-1:500
• Immunogen: Recombinant Mouse Gremlin 1/GREM1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse Gremlin 1/GREM1 (rM Gremlin 1/GREM1). Gremlin 1/GREM1 specific IgG was purified by Mouse Gremlin 1/GREM1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse GREM1 in mosue intestine with rabbit polyclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse GREM1 in mosue heart with rabbit polyclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse GREM1 in mosue brain with rabbit polyclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

Western Blot (WB)

(Anti-GREM1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:21 kDaObserved band size:19 kDa)

Related Product Information for anti-GREM1 antibody

GREM1 belongs to the DAN family. It contains 1 CTCK (C-terminal cystine knot-like) domain. GREM1 is a cysteine knot-secreted protein and acts as an inhibitor in the TGF beta signaling pathway. It inhibits BMP-2, -4, and -7. Inhibition by Grem 1 of BMPs in mice allows the expression of fibroblast growth factors (FGFs) 4 and 8 and Sonic hedgehog (Shh) which are necessary for proper limb development. It interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner. As a cytokine, GREM1 may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. It down-regulates the BMP4 signaling in a dose-dependent manner. It also acts as an inhibitor of monocyte chemotaxis. GREM1 is highly expressed in the small intestine, fetal brain, and colon.

References

Dimitrov BI, et al. (2010) Genomic rearrangements of the GREM1-FMN1 locus cause oligosyndactyly, radio-ulnar synostosis, hearing loss, renal defects syndrome and Cenani--Lenz-like non-syndromic oligosyndactyly. J Med Genet. 47(8):569-74.Heron M, et al. (2011) Genetic variation in GREM1 is a risk factor for fibrosis in pulmonary sarcoidosis. Tissue Antigens. 77(2):112-7.van Vlodrop IJ, et al. (2010) Prognostic significance of Gremlin1 (GREM1) promoter CpG island hypermethylation in clear cell renal cell carcinoma. Am J Pathol. 176(2):575-84.

NCBI and Uniprot Product Information

• NCBI GI #: 6754068
• NCBI GeneID: 23892
• NCBI Accession #: NP_035954.1
• NCBI GenBank Nucleotide #: NM_011824.4
• UniProt Accession #: O70326
• Molecular Weight: 20,710 Da
• NCBI Official Full Name: gremlin-1
• NCBI Official Synonym Full Names: gremlin 1, DAN family BMP antagonist
• NCBI Official Symbol: Grem1
• NCBI Official Synonym Symbols: ld; Drm; Grem; Cktsf1b1
• NCBI Protein Information: gremlin-1
• UniProt Protein Name: Gremlin-1
• Protein Family: Gremlin
• UniProt Gene Name: Grem1
• UniProt Synonym Gene Names: Cktsf1b1; Drm
• UniProt Entry Name: GREM1_MOUSE

Uniprot Description

GREM1: Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Acts as inhibitor of monocyte chemotaxis. Interacts with SLIT1 and SLIT2 in a glycosylation- dependent manner. By high glucose through TGFB1-mediated pathways in mesangial cell. Down-regulated in tumor cell lines. Highly expressed in small intestine, fetal brain and colon. Weakly expressed in brain, ovary, prostate, pancreas and skeletal muscle. In brain found in the region localized around the internal capsule in the large subcortical nuclei, including caudate, putamen, substantia nigra, thalamus and subthalamus. Predominantly expressed in normal cells including neurons, astrocytes and fibroblasts. Belongs to the DAN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: cell surface; extracellular region; extracellular space

Molecular Function: cytokine activity; heparan sulfate proteoglycan binding; receptor agonist activity; transmembrane receptor protein tyrosine kinase activator activity; vascular endothelial growth factor receptor 2 binding

Biological Process: activation of NF-kappaB transcription factor; angiogenesis; cell migration during sprouting angiogenesis; cell morphogenesis; cell-cell signaling; collagen fibril organization; determination of dorsal identity; embryonic limb morphogenesis; endothelial cell migration; limb development; negative regulation of apoptosis; negative regulation of BMP signaling pathway; negative regulation of bone mineralization; negative regulation of bone remodeling; negative regulation of cell growth; negative regulation of chondrocyte differentiation; negative regulation of leukocyte chemotaxis; negative regulation of osteoblast proliferation; negative regulation of transcription, DNA-dependent; organ morphogenesis; positive regulation of angiogenesis; positive regulation of cell proliferation; positive regulation of NF-kappaB import into nucleus; positive regulation of receptor internalization; positive regulation of telomerase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; proximal/distal pattern formation; regulation of focal adhesion formation; regulation of stress-activated MAPK cascade; signal transduction; ureteric bud branching