EPHB1 sirna

EPHB1 siRNA (Mouse)

Cat. Num. AAA8220138

• Gene Names: Ephb1; Elk; Net; Cek6; Elkh; Hek6; AW488255; 9330129L11; C130099E04Rik; ENSMUSG00000074119
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EPHB1; EPHB1 siRNA (Mouse); Ephrin type-B receptor 1; EPHB1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: EPHB1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 943

• Applicable Applications for EPHB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EPHB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EPHB1 sirna

siRNA to inhibit EPHB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 269973848
• NCBI GeneID: 270190
• NCBI Accession #: NP_001161768.1
• NCBI GenBank Nucleotide #: NM_001168296.1
• UniProt Accession #: Q8CBF3; Q3UY27; Q6PG23; Q8CBE2; B1B1C2
• Molecular Weight: 105,294 Da
• NCBI Official Full Name: ephrin type-B receptor 1 isoform 2
• NCBI Official Synonym Full Names: Eph receptor B1
• NCBI Official Symbol: Ephb1
• NCBI Official Synonym Symbols: Elk; Net; Cek6; Elkh; Hek6; AW488255; 9330129L11; C130099E04Rik; ENSMUSG00000074119
• NCBI Protein Information: ephrin type-B receptor 1
• UniProt Protein Name: Ephrin type-B receptor 1
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: Ephb1
• UniProt Entry Name: EPHB1_MOUSE

Uniprot Description

EphB1: a receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-B family: ephrin-B1, -B2 and -B3. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. The ligand-activated form of EphB1 interacts with GRB2, GRB10 and NCK through their respective SH2 domains. Four alternatively spliced isoforms are known.

Protein type: Protein kinase, TK; Membrane protein, integral; Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; Eph family

Cellular Component: filopodium tip; cell projection; membrane; axon; integral to plasma membrane; early endosome membrane; cytoplasm; integral to membrane; plasma membrane; endosome; lipid raft

Molecular Function: transmembrane-ephrin receptor activity; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; protein kinase activity; transferase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; ephrin receptor activity; protein complex binding; kinase activity; axon guidance receptor activity; ATP binding

Biological Process: nervous system development; axon guidance; peptidyl-tyrosine phosphorylation; formation of immunological synapse; protein amino acid autophosphorylation; cranial nerve development; central nervous system projection neuron axonogenesis; optic nerve morphogenesis; protein amino acid phosphorylation; regulation of JNK cascade; cell-substrate adhesion; positive regulation of synaptogenesis; camera-type eye morphogenesis; neurogenesis; establishment of cell polarity; ephrin receptor signaling pathway; angiogenesis; cell adhesion; detection of temperature stimulus involved in sensory perception of pain; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; retinal ganglion cell axon guidance