EphB1 active protein

EphB1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122771

• Gene Names: Ephb1; Elk; Net; Cek6; Elkh; Hek6; AW488255; 9330129L11; C130099E04Rik; ENSMUSG00000074119
• Purity: >98% as determined by SDS-PAGE
• Synonyms: EphB1; EphB1 Protein; Mouse; Recombinant (His Tag); Mouse EphB1/EPHT2 Protein (His Tag); 9330129L11 Protein; AW488255 Protein; C130099E04Rik Protein; Cek6 Protein; Elk Protein; Elkh Protein; ENSMUSG00000074119 Protein; Hek6 Protein; Net Protein; EPH receptor B1; EphB1 active protein

• Host: HEK293 Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu539

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met 18
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse EPHB1 (Q8CBF3-1) extracellular domain (Met 1-Leu 539) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Immobilized mouse EPHB1-His at 10ug/ml (100uL/well) can bind mouse EFNB1-Fc. The EC50 of mouse EFNB1-Fc is 0.04-0.08ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for EphB1 active protein

Background: Ephrin type-B receptor 1, also known as EphB1, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induces various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

Product Categories/Family for EphB1 active protein

Actin Dynamics Signaling Pathway

References

Dalva MB, et al. (2000) EphB receptors interact with NMDA receptors and regulate excitatory synapse formation. Cell. 103(6): 945-56.Takasu MA, et al. (2002) Modulation of NMDA receptor-dependent calcium influx and gene expression through EphB receptors. Science. 295(5554): 491-5.Adams RH, et al. (1999) Roles of ephrinB ligands and EphB receptors in cardiovascular development: demarcation of arterial/venous domains, vascular morphogenesis, and sprouting angiogenesis. Genes Dev. 13(3): 295-306.

NCBI and Uniprot Product Information

• NCBI GI #: 269973846
• NCBI GeneID: 270190
• NCBI Accession #: NP_775623
• NCBI GenBank Nucleotide #: NM_173447.3
• UniProt Accession #: Q8CBF3; Q3UY27; Q6PG23; Q8CBE2; B1B1C2
• Molecular Weight: 109,881 Da
• NCBI Official Full Name: ephrin type-B receptor 1 isoform 1
• NCBI Official Synonym Full Names: Eph receptor B1
• NCBI Official Symbol: Ephb1
• NCBI Official Synonym Symbols: Elk; Net; Cek6; Elkh; Hek6; AW488255; 9330129L11; C130099E04Rik; ENSMUSG00000074119
• NCBI Protein Information: ephrin type-B receptor 1
• UniProt Protein Name: Ephrin type-B receptor 1
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: Ephb1
• UniProt Entry Name: EPHB1_MOUSE