Western Blot (WB)
(Western Blot analysis of HeLa, 293 cells using CD148 Polyclonal Antibody.)
Rabbit anti-Human CD148 Polyclonal Antibody | anti-CD148 antibody
CD148 Polyclonal Antibody
ELISA: 1:20000
Not yet tested in other applications.
Western Blot (WB)
(Western Blot analysis of HeLa, 293 cells using CD148 Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of HeLa, 293 cells using CD148 Polyclonal Antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
PTPRJ: Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, RET (variant MEN2A), KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation. Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling. Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor protein phosphatase, tyrosine; Motility/polarity/chemotaxis; EC 3.1.3.48
Chromosomal Location of Human Ortholog: 11p11.2
Cellular Component: nucleoplasm; cell surface; integral to plasma membrane; plasma membrane; immunological synapse; intercellular junction; cell junction
Molecular Function: protein binding; gamma-catenin binding; phosphoric monoester hydrolase activity; beta-catenin binding; platelet-derived growth factor receptor binding; mitogen-activated protein kinase binding; protein tyrosine phosphatase activity; protein kinase binding
Biological Process: regulation of cell adhesion; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of MAP kinase activity; negative regulation of protein kinase B signaling cascade; positive regulation of cell adhesion; heart development; negative regulation of T cell receptor signaling pathway; platelet-derived growth factor receptor signaling pathway; negative regulation of cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of focal adhesion formation; positive chemotaxis; negative regulation of vascular permeability; oligodendrocyte differentiation; negative regulation of cell growth; blood coagulation; vasculogenesis; negative regulation of cell migration
Disease: Colorectal Cancer
Research Articles on CD148
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Product Notes
The CD148 ptprj (Catalog #AAA9405198) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD148 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD148 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Western Blot: 1:500 - 1:2000 ELISA: 1:20000 Not yet tested in other applications. Researchers should empirically determine the suitability of the CD148 ptprj for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD148, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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