Western Blot (WB)
(All lanes : Anti-Ephb1 Antibody (Center) at 1:1000 dilutionLane 1: mouse brain lysatesLane 2: mouse heart lysatesLane 3: rat brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 110 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit Ephb1 Polyclonal Antibody | anti-Ephb1 antibody
Mouse Ephb1 Antibody (Center)
Western Blot (WB)
(All lanes : Anti-Ephb1 Antibody (Center) at 1:1000 dilutionLane 1: mouse brain lysatesLane 2: mouse heart lysatesLane 3: rat brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 110 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-Ephb1 Antibody (Center) at 1:1000 dilutionLane 1: mouse brain lysatesLane 2: mouse heart lysatesLane 3: rat brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 110 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western blot analysis of lysates from A431, mouse NIH/3T3 cell line, mouse brain, rat brain tissue lysate(from left to right), using Ephb1 Antibody (Center). MBS9203757 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from A431, mouse NIH/3T3 cell line, mouse brain, rat brain tissue lysate(from left to right), using Ephb1 Antibody (Center). MBS9203757 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009).
Stein E.,et al.J. Biol. Chem. 273:1303-1308(1998).
Torres R.,et al.Neuron 21:1453-1463(1998).
Han D.C.,et al.J. Biol. Chem. 277:45655-45661(2002).
NCBI and Uniprot Product Information
Uniprot Description
EphB1: a receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-B family: ephrin-B1, -B2 and -B3. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. The ligand-activated form of EphB1 interacts with GRB2, GRB10 and NCK through their respective SH2 domains. Four alternatively spliced isoforms are known.
Protein type: Protein kinase, tyrosine (receptor); Kinase, protein; Protein kinase, TK; EC 2.7.10.1; Membrane protein, integral; TK group; Eph family
Cellular Component: filopodium tip; cell projection; membrane; early endosome membrane; axon; integral to plasma membrane; cytoplasm; plasma membrane; integral to membrane; endosome; lipid raft
Molecular Function: transmembrane-ephrin receptor activity; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; protein kinase activity; transferase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; ephrin receptor activity; protein complex binding; kinase activity; axon guidance receptor activity; ATP binding
Biological Process: axon guidance; nervous system development; peptidyl-tyrosine phosphorylation; formation of immunological synapse; protein amino acid autophosphorylation; cranial nerve development; central nervous system projection neuron axonogenesis; optic nerve morphogenesis; protein amino acid phosphorylation; regulation of JNK cascade; cell-substrate adhesion; positive regulation of synaptogenesis; camera-type eye morphogenesis; neurogenesis; establishment of cell polarity; ephrin receptor signaling pathway; angiogenesis; cell adhesion; detection of temperature stimulus involved in sensory perception of pain; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; retinal ganglion cell axon guidance
Research Articles on Ephb1
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Product Notes
The Ephb1 ephb1 (Catalog #AAA9203757) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mouse Ephb1 Antibody (Center) reacts with Human, mouse, rat (Predicted Reactivity: Chicken) and may cross-react with other species as described in the data sheet. AAA Biotech's Ephb1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Ephb1 ephb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ephb1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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