CCL12 sirna

CCL12 siRNA (Mouse)

Cat. Num. AAA827879

• Gene Names: Ccl12; MCP-5; Scya12
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CCL12; CCL12 siRNA (Mouse); MCP5; SCYA12; C-C motif chemokine 12; MCP-1-related chemokine; Monocyte chemoattractant protein 5; Monocyte chemotactic protein 5; MCP-5; Small-inducible cytokine A12; CCL12 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CCL12 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 104

• Applicable Applications for CCL12 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CCL12 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CCL12 sirna

siRNA to inhibit CCL12 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 119226206
• NCBI GeneID: 20293
• NCBI Accession #: NP_035461.2
• NCBI GenBank Nucleotide #: NM_011331.2
• UniProt Accession #: Q62401; Q9QYD6
• Molecular Weight: 11,659 Da
• NCBI Official Full Name: C-C motif chemokine 12
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 12
• NCBI Official Symbol: Ccl12
• NCBI Official Synonym Symbols: MCP-5; Scya12
• NCBI Protein Information: C-C motif chemokine 12
• UniProt Protein Name: C-C motif chemokine 12
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl12
• UniProt Synonym Gene Names: Mcp5; Scya12; MCP-5
• UniProt Entry Name: CCL12_MOUSE

Uniprot Description

CCL2: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide; Chemokine

Cellular Component: extracellular space; extracellular region; intracellular

Molecular Function: CCR2 chemokine receptor binding; chemokine activity; cytokine activity

Biological Process: positive regulation of leukocyte migration; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; cytoskeleton organization and biogenesis; chemotaxis; macrophage chemotaxis; monocyte chemotaxis; regulation of cell shape; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; immune response; negative regulation of neuron apoptosis; angiogenesis; inflammatory response; astrocyte cell migration