Rabbit HSP10 Polyclonal Antibody | anti-HSP10 antibody

HSP10 (K80) Polyclonal Antibody

Cat. Num. AAA3000604

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: HSP10; Polyclonal Antibody; HSP10 (K80) Polyclonal Antibody; Hsp10; 10 kDa chaperonin; Chaperonin 10; CPN10; Early-pregnancy factor; EPF; HSPE1; anti-HSP10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HSP10 (K80) polyclonal antibody detects endogenous levels of HSP10 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 102

• Applicable Applications for anti-HSP10 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 30-100 of Human HSP10.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of HSP10 (K80) pAb at 1:500 dilutionLane1:A375 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:The Kidney tissue lysate of Mouse (40ug) Lane5:The Kidney tissue lysate of Rat (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of HSP10 (K80) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-HSP10 antibody

The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent polypeptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The low molecular weight family includes HSP 10, HSP 20, HSP 27 (Heme Oxygenase 1), HSP 32 and HSP 40. Chaperonins are ubiquitous, indispensable proteins that facilitate protein folding in an ATP-dependent manner, enhancing the yield of properly folded substrate protein under conditions where spontaneous folding does not occur. Chaperonins are typified by the E. coli heat-shock proteins GroEL and GroES (HSP 10). HSP 10 is a heptameric ring of identical 10 kDa subunits that binds to each end of GroEL to form a symmetric, functional heterodimer.

NCBI and Uniprot Product Information

• NCBI GI #: 4504523
• NCBI Accession #: NP_002148.1
• NCBI GenBank Nucleotide #: NP_002148.1
• UniProt Accession #: P61604
• Molecular Weight: ~ 10kDa
• NCBI Official Full Name: 10 kDa heat shock protein, mitochondrial
• UniProt Protein Name: 10 kDa heat shock protein, mitochondrial
• Protein Family: 10 kDa heat shock protein
• UniProt Gene Name: HSPE1
• UniProt Synonym Gene Names: Hsp10; CPN10; EPF
• UniProt Entry Name: CH10_HUMAN

Uniprot Description

HSPE1: Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. Belongs to the GroES chaperonin family.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: membrane; mitochondrion; mitochondrial matrix

Molecular Function: protein binding; chaperone binding; unfolded protein binding; ATP binding

Biological Process: caspase activation; osteoblast differentiation; protein folding; response to unfolded protein