Human Proenkephalin-A ELISA Kit | PENK elisa kit

Human Proenkephalin-A ELISA Kit

Cat. Num. AAA2885756

• Gene Names: PENK; PE; PENK-A
• Reactivity: Human
• Synonyms: Proenkephalin-A; Human Proenkephalin-A ELISA Kit; PENK; PENK elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human Proenkephalin-A
• Sequence Length: 267

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 78-5000 pg/mL
• Sensitivity: <31 pg/mL
• Intra-assay Precision: Intra-Assay CV: <=5.2%
• Inter-assay Precision: Inter-Assay CV: <=8.6%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PENK elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 208879446
• NCBI GeneID: 5179
• NCBI Accession #: NP_001129162.1
• NCBI GenBank Nucleotide #: NM_001135690.2
• UniProt Accession #: P01210; Q6FHC6; Q6FHE6; B2RC23
• Molecular Weight: 30,787 Da
• NCBI Official Full Name: proenkephalin-A preproprotein
• NCBI Official Synonym Full Names: proenkephalin
• NCBI Official Symbol: PENK
• NCBI Official Synonym Symbols: PE; PENK-A
• NCBI Protein Information: proenkephalin-A
• UniProt Protein Name: Proenkephalin-A
• Protein Family: Proenkephalin
• UniProt Gene Name: PENK
• UniProt Synonym Gene Names: OGF
• UniProt Entry Name: PENK_HUMAN

NCBI Description

This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the pentapeptide opioids Met-enkephalin and Leu-enkephalin, which are stored in synaptic vesicles, then released into the synapse where they bind to mu- and delta-opioid receptors to modulate the perception of pain. Other non-opioid cleavage products may function in distinct biological activities. [provided by RefSeq, Jul 2015]

Uniprot Description

PENK: Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. Belongs to the opioid neuropeptide precursor family.

Protein type: Secreted, signal peptide; Secreted; Hormone

Chromosomal Location of Human Ortholog: 8q23-q24

Cellular Component: dendrite; extracellular region; nerve terminal; perikaryon

Molecular Function: neuropeptide hormone activity; opioid peptide activity; protein binding

Biological Process: aging; behavioral fear response; general adaptation syndrome, behavioral process; glial cell proliferation; neuropeptide signaling pathway; osteoblast differentiation; response to calcium ion; response to estradiol stimulus; response to ethanol; response to hypoxia; response to lipopolysaccharide; response to morphine; response to nicotine; response to radiation; response to toxin; sensory perception of pain; signal transduction; startle response