HIVEP1 sirna

HIVEP1 siRNA (Human)

Cat. Num. AAA8226079

• Gene Names: HIVEP1; GAAP; ZAS1; CIRIP; MBP-1; ZNF40; CRYBP1; ZNF40A; PRDII-BF1; Schnurri-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HIVEP1; HIVEP1 siRNA (Human); ZNF40; Zinc finger protein 40; Cirhin interaction protein; CIRIP; Gate keeper of apoptosis-activating protein; GAAP; Human immunodeficiency virus type I enhancer-binding protein 1; HIV-EP1; Major histocompatibility complex-binding protein 1; MBP-1; Positive regulatory domain II-binding factor 1; PRDII-BF1; HIVEP1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HIVEP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 716

• Applicable Applications for HIVEP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HIVEP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HIVEP1 sirna

siRNA to inhibit HIVEP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 613410170
• NCBI GeneID: 3096
• NCBI Accession #: NP_002105.3
• NCBI GenBank Nucleotide #: NM_002114.3
• UniProt Accession #: P15822; Q14122; Q5MPB1; Q5VW60; B2RTU3
• Molecular Weight: 77,538 Da
• NCBI Official Full Name: zinc finger protein 40
• NCBI Official Synonym Full Names: human immunodeficiency virus type I enhancer binding protein 1
• NCBI Official Symbol: HIVEP1
• NCBI Official Synonym Symbols: GAAP; ZAS1; CIRIP; MBP-1; ZNF40; CRYBP1; ZNF40A; PRDII-BF1; Schnurri-1
• NCBI Protein Information: zinc finger protein 40
• UniProt Protein Name: Zinc finger protein 40
• Protein Family: Zinc finger protein
• UniProt Gene Name: HIVEP1
• UniProt Synonym Gene Names: ZNF40; CIRIP; GAAP; HIV-EP1; MBP-1; PRDII-BF1
• UniProt Entry Name: ZEP1_HUMAN

NCBI Description

This gene encodes a transcription factor belonging to the ZAS family, members of which are large proteins that contain a ZAS domain - a modular protein structure consisting of a pair of C2H2 zinc fingers with an acidic-rich region and a serine/threonine-rich sequence. These proteins bind specifically to the DNA sequence motif, GGGACTTTCC, found in the enhancer elements of several viral promoters, including human immunodeficiency virus (HIV), and to related sequences found in the enhancer elements of a number of cellular promoters. This protein binds to this sequence motif, suggesting a role in the transcriptional regulation of both viral and cellular genes. [provided by RefSeq, Oct 2011]

Uniprot Description

HIVEP1: This protein specifically binds to the DNA sequence 5'- GGGACTTTCC-3' which is found in the enhancer elements of numerous viral promoters such as those of SV40, CMV, or HIV-1. In addition, related sequences are found in the enhancer elements of a number of cellular promoters, including those of the class I MHC, interleukin-2 receptor, and interferon-beta genes. It may act in T-cell activation. Involved in activating HIV-1 gene expression. Isoform 2 and isoform 3 also bind to the IPCS (IRF1 and p53 common sequence) DNA sequence in the promoter region of interferon regulatory factor 1 and p53 genes and are involved in transcription regulation of these genes. Isoform 2 does not activate HIV-1 gene expression. Isoform 2 and isoform 3 may be involved in apoptosis. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 6p24-p22.3

Cellular Component: nucleoplasm; mitochondrion; nucleus

Molecular Function: protein binding; DNA binding; metal ion binding

Biological Process: transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter