Testing Data
(Recombinant Human CXCL11/I‑TAC chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CXCL11/I‑TAC (7ng/ml) is neutralized (green line) by increasing concentrations of MBS600325. The ND50 is typically 0.3-1.5µg/ml.)
Mouse anti-Human CXCR3 Monoclonal Antibody | anti-CXCR3 antibody
CXCR3 (CD183)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: Neutralization (ND50): ~0.3-1.5ug/ml in the presence of 7ng/ml of recombinant human CXCL11/l-TAC, using the human CXCR3 transfected BaF/3 cells in a chemotaxis assay.
The exact concentration required to neutralize human cell surface CXCR3 activity is dependent on the ligand concentration as well as the number of CXCR3 receptors present on the cell surface.
Flow Cytometry: 2.5x10e6 cells using whole human blood lymphocytes.
Immunohistochemistry: 8-25ug/ml detected CXCR3 in immersion fixed paraffin-embedded sections of human tonsil.
Testing Data
(Recombinant Human CXCL11/I‑TAC chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CXCL11/I‑TAC (7ng/ml) is neutralized (green line) by increasing concentrations of MBS600325. The ND50 is typically 0.3-1.5µg/ml.)
Testing Data
(Recombinant Human CXCL11/I‑TAC chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CXCL11/I‑TAC (7ng/ml) is neutralized (green line) by increasing concentrations of MBS600325. The ND50 is typically 0.3-1.5µg/ml.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of CXCR3 in immersion fixed paraffin-embedded section of human tonsil using MBS600325 at 3ug/ml with overnight incubation at 4°C. HRP-conjugated anti-mouse IgG and DAB staining were used for visualization. The tissue was counterstained with hematoxylin (blue).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of CXCR3 in immersion fixed paraffin-embedded section of human tonsil using MBS600325 at 3ug/ml with overnight incubation at 4°C. HRP-conjugated anti-mouse IgG and DAB staining were used for visualization. The tissue was counterstained with hematoxylin (blue).)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of HEK293 cells transfected with human CXCR3 (upper histogram) or with irrelevant transfectants and eGFP (lower histogram) were stained with MBS600325 followed by APC-conjugated anti-mouse IgG secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of HEK293 cells transfected with human CXCR3 (upper histogram) or with irrelevant transfectants and eGFP (lower histogram) were stained with MBS600325 followed by APC-conjugated anti-mouse IgG secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716). [provided by RefSeq]
Uniprot Description
CXCR3: Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC). Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR
Chromosomal Location of Human Ortholog: Xq13
Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane
Molecular Function: C-X-C chemokine binding; chemokine binding; chemokine receptor activity; receptor activity; C-X-C chemokine receptor activity
Biological Process: positive regulation of chemotaxis; apoptosis; calcium-mediated signaling; positive regulation of cAMP metabolic process; chemotaxis; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; positive regulation of angiogenesis; negative regulation of angiogenesis; cell surface receptor linked signal transduction; negative regulation of endothelial cell proliferation; regulation of leukocyte migration; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; positive regulation of release of sequestered calcium ion into cytosol; cell motility; cell adhesion; inflammatory response
Research Articles on CXCR3
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Product Notes
The CXCR3 cxcr3 (Catalog #AAA600325) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CXCR3 (CD183) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CXCR3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Neutralization. Suitable for use in Neutralization, Flow Cytometry and Immunohistochemistry. Dilution: Neutralization (ND50): ~0.3-1.5ug/ml in the presence of 7ng/ml of recombinant human CXCL11/l-TAC, using the human CXCR3 transfected BaF/3 cells in a chemotaxis assay. The exact concentration required to neutralize human cell surface CXCR3 activity is dependent on the ligand concentration as well as the number of CXCR3 receptors present on the cell surface. Flow Cytometry: 2.5x10e6 cells using whole human blood lymphocytes. Immunohistochemistry: 8-25ug/ml detected CXCR3 in immersion fixed paraffin-embedded sections of human tonsil. Researchers should empirically determine the suitability of the CXCR3 cxcr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CXCR3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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