Human HIVEP1 (Zinc finger protein 40) ELISA Kit | HIVEP1 elisa kit

Human HIVEP1 (Zinc finger protein 40) ELISA Kit

Cat. Num. AAA7612344

• Gene Names: HIVEP1; GAAP; ZAS1; CIRIP; MBP-1; ZNF40; CRYBP1; ZNF40A; PRDII-BF1; Schnurri-1
• Reactivity: Human
• Synonyms: HIVEP1 (Zinc finger protein 40); Human HIVEP1 (Zinc finger protein 40) ELISA Kit; HIVEP1; Zinc finger protein 40; Cirhin interaction protein; Gate keeper of apoptosis-activating protein; CIRIP; GAAP; Human immunodeficiency virus type I enhancer-binding protein 1; HIV-EP1; Major histocompatibility complex-binding protein 1; MBP-1; Positive regulatory domain II-binding factor 1; PRDII-BF1; ZNF40; HIVEP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of HIVEP1. No significant cross-reactivity or interference between HIVEP1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HIVEP1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 613410169
• NCBI GeneID: 3096
• NCBI Accession #: NP_002105.3
• NCBI GenBank Nucleotide #: NM_002114
• UniProt Accession #: P15822; Q14122; Q5MPB1; Q5VW60; B2RTU3
• Molecular Weight: 77,538 Da
• NCBI Official Full Name: Homo sapiens human immunodeficiency virus type I enhancer binding protein 1 (HIVEP1), mRNA
• NCBI Official Synonym Full Names: human immunodeficiency virus type I enhancer binding protein 1
• NCBI Official Symbol: HIVEP1
• NCBI Official Synonym Symbols: GAAP; ZAS1; CIRIP; MBP-1; ZNF40; CRYBP1; ZNF40A; PRDII-BF1; Schnurri-1
• NCBI Protein Information: zinc finger protein 40; cirhin interaction protein; gate keeper of apoptosis-activating protein; major histocompatibility complex binding protein 1; positive regulatory domain II binding factor 1
• UniProt Protein Name: Zinc finger protein 40
• Protein Family: Zinc finger protein
• UniProt Gene Name: HIVEP1
• UniProt Synonym Gene Names: ZNF40; CIRIP; GAAP; HIV-EP1; MBP-1; PRDII-BF1
• UniProt Entry Name: ZEP1_HUMAN

NCBI Description

This gene encodes a transcription factor belonging to the ZAS family, members of which are large proteins that contain a ZAS domain - a modular protein structure consisting of a pair of C2H2 zinc fingers with an acidic-rich region and a serine/threonine-rich sequence. These proteins bind specifically to the DNA sequence motif, GGGACTTTCC, found in the enhancer elements of several viral promoters, including human immunodeficiency virus (HIV), and to related sequences found in the enhancer elements of a number of cellular promoters. This protein binds to this sequence motif, suggesting a role in the transcriptional regulation of both viral and cellular genes. [provided by RefSeq, Oct 2011]