Spleen Tyrosine Kinase (SYK) Recombinant Protein | SYK recombinant protein

Recombinant Spleen Tyrosine Kinase (SYK)

Cat. Num. AAA2010441

• Gene Names: Syk; Sykb
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Spleen Tyrosine Kinase (SYK); Recombinant Spleen Tyrosine Kinase (SYK); SYK recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- E VYESPYADPE EIRPKEVYLD RSLLTLEDNE LGSGNFGTVK KGYYQMKKVV KTVAVKILKN EANDPALKDE LLAEANVMQQ LDNPYIVRMI GICEAESWML VMEMAELGPL NKYLQQNRHI KDKNIIELVH QVSMGMKYLE ESNFVHRDLA ARNVLLVTQH YAKISDFGLS KALRADENYY KAQTHGKWPV KWYAPECINY YKFSSKSDVW SFGVLMWEAF SYGQKPYRGM KGSEVTAMLE KGERMGCPAG CPREMYDLMN LCWTYDV
• Sequence Length: 629

• Applicable Applications for SYK recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Glu340~Val607 (Accession # P48025) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 312176429
• NCBI GeneID: 20963
• NCBI Accession #: NP_001185906.1
• NCBI GenBank Nucleotide #: NM_001198977.1
• UniProt Accession #: P48025
• Molecular Weight: 34.8kDa
• NCBI Official Full Name: tyrosine-protein kinase SYK
• NCBI Official Synonym Full Names: spleen tyrosine kinase
• NCBI Official Symbol: Syk
• NCBI Official Synonym Symbols: Sykb
• NCBI Protein Information: tyrosine-protein kinase SYK
• UniProt Protein Name: Tyrosine-protein kinase SYK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Syk
• UniProt Synonym Gene Names: ptk72; Sykb
• UniProt Entry Name: KSYK_MOUSE

Uniprot Description

Syk: a cytoplasmic tyrosine kinase of the SYK family containing two SH2 domains. Plays a central role in the B cell receptor (BCR) response. An upstream activator of the PI3K, PLCgamma2, and Rac/cdc42 pathways in the BCR response. Required for the sequential events of Fc gamma IIa receptor-mediated phagocytosis. Expression highest in murine spleen, heart, mammary gland and thymus. Two splice variant isoforms have been described.

Protein type: EC 2.7.10.2; Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (non-receptor); TK group; Syk family

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; protein complex; membrane; cell; cytoplasm; plasma membrane; intracellular; cytoplasmic vesicle; nucleus; B cell receptor complex

Molecular Function: protein domain specific binding; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; protein kinase binding; protein kinase activity; transferase activity; integrin binding; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; kinase activity; ATP binding; receptor binding

Biological Process: peptidyl-tyrosine phosphorylation; transcription factor import into nucleus; activation of MAPK activity; protein amino acid autophosphorylation; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of interleukin-3 biosynthetic process; positive regulation of calcium-mediated signaling; regulation of phagocytosis; enzyme linked receptor protein signaling pathway; protein amino acid phosphorylation; activation of JNK activity; serotonin secretion; leukocyte adhesion; B cell receptor signaling pathway; cell surface receptor linked signal transduction; regulation of transcription factor activity; positive regulation of bone resorption; beta selection; angiogenesis; positive regulation of gamma-delta T cell differentiation; integrin-mediated signaling pathway; neutrophil chemotaxis; adaptive immune response; regulation of immune response; cell migration; immune system process; regulation of superoxide release; positive regulation of cell adhesion mediated by integrin; positive regulation of mast cell degranulation; regulation of neutrophil degranulation; leukotriene biosynthetic process; G-protein coupled receptor protein signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation; peptidyl-serine phosphorylation; neutrophil activation during immune response; leukocyte activation during immune response; serotonin secretion by platelet; macrophage activation during immune response; defense response to bacterium; positive regulation of B cell differentiation; blood vessel morphogenesis; innate immune response; positive regulation of alpha-beta T cell proliferation; lymph vessel development; phosphorylation; positive regulation of alpha-beta T cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cytokine secretion