TXK Tyrosine Kinase (TXK) Recombinant Protein | TXK recombinant protein

Recombinant TXK Tyrosine Kinase (TXK)

Cat. Num. AAA2010541

• Gene Names: Txk; Rlk; Btkl; PTK4; PTK-RL-18; A130089B16Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: TXK Tyrosine Kinase (TXK); Recombinant TXK Tyrosine Kinase (TXK); TXK recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-HLGEW RAHIPVAIKA INEGSMSEED FIEEAKVMMK LSHSRLVQLY GVCIQQKPLY IVTEFMENGC LLDYLRERKG QLQKALLLSM CQDICEGMAY LERSCYIHRD LAARNCLVSS ACVVKISDFG MARYVLDDEY ISSSGAKFPV KWCPPEVFHF NKYSSKSDVW SFGVLMWEVF TEGKMPFENK SNLQVVEAIS QGFRLYRPHL APMTIYRVMY SCWHESPKG
• Sequence Length: 527

• Applicable Applications for TXK recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: His286~Gly509 (Accession # P42682) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 170295811
• NCBI GeneID: 22165
• NCBI Accession #: NP_001116226.1
• NCBI GenBank Nucleotide #: NM_001122754.2
• UniProt Accession #: P42682
• Molecular Weight: 29.7kDa
• NCBI Official Full Name: tyrosine-protein kinase TXK isoform 1
• NCBI Official Synonym Full Names: TXK tyrosine kinase
• NCBI Official Symbol: Txk
• NCBI Official Synonym Symbols: Rlk; Btkl; PTK4; PTK-RL-18; A130089B16Rik
• NCBI Protein Information: tyrosine-protein kinase TXK; resting lymphocyte kinase
• UniProt Protein Name: Tyrosine-protein kinase TXK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Txk
• UniProt Synonym Gene Names: Rlk
• UniProt Entry Name: TXK_MOUSE

Uniprot Description

TXK: Non-receptor tyrosine kinase that plays a redundant role with ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T- cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of TXK to the cell membrane, where it is phosphorylated at Tyr-420. Phosphorylation leads to TXK full activation. Contributes also to signaling from many receptors and participates in multiple downstream pathways, including regulation of the actin cytoskekleton. Like ITK, can phosphorylate PLCG1, leading to its localization in lipid rafts and activation, followed by subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. With PARP1 and EEF1A1, TXK forms a complex that acts as a T-helper 1 (Th1) cell- specific transcription factor and binds the promoter of IFNG to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Phosphorylates both PARP1 and EEF1A1. Phosphorylates also key sites in LCP2 leading to the up-regulation of Th1 preferred cytokine IL-2. Phosphorylates 'Tyr- 201' of CTLA4 which leads to the association of PI-3 kinase with the CTLA4 receptor. Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.

Protein type: Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; Kinase, protein; TK group; Tec family

Cellular Component: extrinsic to internal side of plasma membrane; membrane; cytoplasm; plasma membrane; nucleus

Molecular Function: transferase activity; DNA binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: transcription from RNA polymerase II promoter; adaptive immune response; transcription, DNA-dependent; immune system process; protein amino acid autophosphorylation; cytokine production; T cell receptor signaling pathway; protein amino acid phosphorylation; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; positive regulation of interferon-gamma production; interleukin-4 production; regulation of transcription, DNA-dependent; phospholipase C activation; interferon-gamma production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; NK T cell differentiation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway