STAT5 recombinant protein

STAT5 recombinant protein

Cat. Num. AAA515346

• Gene Names: STAT5A; MGF; STAT5
• Applications: Western Blot
• Purity: >75%
• Synonyms: STAT5; STAT5 recombinant protein; MGF; STAT5A

• Host: Sf9 insect cells
• Purity/Purification: >75%
• Form/Format: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 2mM DTT, 25% glycerol.
• Concentration: 0.1ug/ul (varies by lot)
• Sequence Length: 4314

• Applicable Applications for STAT5 recombinant protein: Kinase Assay, Western Blot (WB)
• Preparation and Storage: Ships on dry ice. Store at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.; Shipping: Ships on Dry Ice.

SDS-PAGE

(The purity of STAT5 was determined to be >90% by densitometry. Approx. MW 92kDa.)

Related Product Information for STAT5 recombinant protein

Recombinant full-length human STAT5 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Scientific Background: STAT5 (signal transducers and activators of transcription 5) is a member of the STAT family and this protein is phosphorylated by receptor associated protein kinases in response to cytokines and growth factors (1). The differentiation of T helper (Th) cells is regulated by members of the STAT family of signaling molecules. In BCR-ABL-positive cells, STAT5 is constitutively activated by tyrosine phosphorylation. STAT5 activation results in upregulation of Bcl-XL and increased resistance to induction of apoptosis. STAT5 is involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6 beta-hydroxylase (2).

Product Categories/Family for STAT5 recombinant protein

Transcription Proteins; Signaling Proteins - Transcription Proteins

References

1. Wang, D. et al: Naturally occurring dominant negative variants of Stat5. Molec. Cell Biol. 16: 6141-6148, 1996.
2. A Subramanian et al: STAT 5 and NF-Y are involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6beta-hydroxylase. Nucleic Acids Res. 1998; 26(9): 2173 -2178.

NCBI and Uniprot Product Information

• NCBI GI #: 221316717
• NCBI GeneID: 6776
• NCBI Accession #: NM_0031512
• NCBI GenBank Nucleotide #: NM_003152
• UniProt Accession #: P42229; Q1KLZ6
• NCBI Official Full Name: Homo sapiens signal transducer and activator of transcription 5A (STAT5A), mRNA
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 5A
• NCBI Official Symbol: STAT5A
• NCBI Official Synonym Symbols: MGF; STAT5
• NCBI Protein Information: signal transducer and activator of transcription 5A
• UniProt Protein Name: Signal transducer and activator of transcription 5A
• UniProt Gene Name: STAT5A
• UniProt Synonym Gene Names: STAT5
• UniProt Entry Name: STA5A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation. Ref.7 Ref.12

Subunit structure: Forms a homodimer or a heterodimer with a related family member. Binds NR3C1

By similarity. Interacts with NCOA1 and SOCS7. Interacts with ERBB4. Ref.8 Ref.12 Ref.13

Subcellular location: Cytoplasm. Nucleus. Note: Translocated into the nucleus in response to phosphorylation. Ref.12

Post-translational modification: Tyrosine phosphorylated in response to KITLG/SCF, IL2, IL3, IL7, IL15, CSF2/GMCSF, GH1, PRL, EPO and THPO. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity

By similarity. Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2 at Tyr-694. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway. Ref.7 Ref.9 Ref.10 Ref.17ISGylated

By similarity.

Sequence similarities: Belongs to the transcription factor STAT family.Contains 1 SH2 domain.