S100A13 recombinant protein

Recombinant Human S100A13 Protein

Cat. Num. AAA2545976

• Purity: > 98 % as determined by SDS-PAGE
• Synonyms: S100A13; Recombinant Human S100A13 Protein; OTTHUMP00000034802; S100 calcium-binding protein A13; protein S100-A13; S100A13 recombinant protein

• Host: E Coli
• Purity/Purification: > 98 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 100mM NaCl, pH 8.0
• Sequence Length: 98

• Application Notes: The recombinant human S100A13 consisting of 98 amino acids and has a calculated molecular mass of 11.6 kDa. It migrates as an 13 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met 1
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for S100A13 recombinant protein

Background: S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response.  Protein S100-A13, also known as S100 calcium-binding protein A13, is a member of the S-100 family. It contains two EF-hand domains. S100A13 binds two calcium ions per subunit and one copper ion. Binding of one copper ion does not interfere with calcium binding. S100A13 is required for the copper-dependent stress-induced export of IL1A and FGF1. The calcium-free protein binds to lipid vesicles containing phosphatidylserine, but not to vesicles containing phosphatidylcholine. S100A13 plays a role in the export of proteins that lack a signal peptide and are secreted by an alternative pathway.

Description: A DNA sequence encoding the native human S100A13 (NP_001019381.1) (Met 1-Lys 98) was expressed.

NCBI and Uniprot Product Information

• NCBI GI #: 66737368
• NCBI GeneID: 6284
• NCBI Accession #: NP_001019381.1
• NCBI GenBank Nucleotide #: NM_001024210.1
• UniProt Accession #: Q99584; Q52PI9; Q6FGF8
• Molecular Weight: 11,471 Da
• NCBI Official Full Name: protein S100-A13
• NCBI Official Synonym Full Names: S100 calcium binding protein A13
• NCBI Official Symbol: S100A13
• NCBI Protein Information: protein S100-A13
• UniProt Protein Name: Protein S100-A13
• Protein Family: Protein
• UniProt Gene Name: S100A13

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is widely expressed in various types of tissues with a high expression level in thyroid gland. In smooth muscle cells, this protein co-expresses with other family members in the nucleus and in stress fibers, suggesting diverse functions in signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

S100A13: Plays a role in the export of proteins that lack a signal peptide and are secreted by an alternative pathway. Binds two calcium ions per subunit. Binds one copper ion. Binding of one copper ion does not interfere with calcium binding. Required for the copper-dependent stress-induced export of IL1A and FGF1. The calcium-free protein binds to lipid vesicles containing phosphatidylserine, but not to vesicles containing phosphatidylcholine. Belongs to the S-100 family.

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cytoplasm; cytosol; extracellular space; nucleus; perinuclear region of cytoplasm

Molecular Function: calcium ion binding; copper ion binding; fibroblast growth factor binding; protein binding; protein homodimerization activity; RAGE receptor binding; zinc ion binding

Biological Process: cytokine secretion; interleukin-1 alpha secretion; positive regulation of I-kappaB kinase/NF-kappaB cascade