SDS-PAGE
(The purity was determined to be >80% by densitometry. Approx. MW 91 kDa)
HSP90 beta recombinant protein
HSP90 beta recombinant protein
SDS-PAGE
(The purity was determined to be >80% by densitometry. Approx. MW 91 kDa)
SDS-PAGE
(The purity was determined to be >80% by densitometry. Approx. MW 91 kDa)
Scientific Background: HSP90β is a member of the HSP90 family of proteins which are important molecular chaperones involved in signal transduction, cell cycle control, stress management, and folding, degradation, and transport of proteins (1). HSP90 proteins have been found in a variety of organisms suggesting that they are ancient and conserved. HSP90 binds to client proteins (such as steroid receptors, AKT, Bcr-Abl, Apaf-1, survivin, cyclin dependent kinases) and acts as a molecular chaperone. Failure of Hsp90 chaperone activity leads to misfolding of client proteins, which leads to ubiquitination and proteasome degradation, and thus deregulation of cellular homeostasis (2).
2. Georgakis, G.V. et al: Heat-shock protein 90 inhibitors in cancer therapy: 17AAG and beyond. Future Oncol. 2005 Apr;1(2):273-81.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012]
Uniprot Description
Function: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Ref.22 Ref.29
Subunit structure: Homodimer. Interacts with p53/TP53
By similarity. Forms a complex with CDK6 and Hsp90/HSP90AB1. Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer. Interacts with CHORDC1 and DNAJC7
By similarity. Interacts with FKBP4. Ref.17 Ref.22 Ref.25
Subcellular location: Cytoplasm. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.17 Ref.21
Domain: The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins
By similarity.
Post-translational modification: Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro). Ref.24ISGylated. Ref.18S-nitrosylated; negatively regulates the ATPase activity
Probable.
Sequence similarities: Belongs to the heat shock protein 90 family.
Sequence caution: The sequence AAD14062.3 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence CAB66478.1 differs from that shown. Reason: Frameshift at position 709.
Research Articles on HSP90 beta
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Product Notes
The HSP90 beta hsp90ab1 (Catalog #AAA515103) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant full length human HSP90beta was expressed by baculovirus in Sf9 insect cells using C-terminal His tag. AAA Biotech's HSP90 beta can be used in a range of immunoassay formats including, but not limited to, ATPase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the HSP90 beta hsp90ab1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP90 beta, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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