ST6GAL1 recombinant protein

ST6GAL1, 27-406aa, Human, His tag, Baculovirus

Cat. Num. AAA206279

• Gene Names: ST6GAL1; ST6N; SIAT1; ST6GalI
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: ST6GAL1; 27-406aa; Human; His tag; Baculovirus; Beta-galactoside alpha-2; 6-sialyltransferase 1; SIAT1; ST6GalI; ST6N; ST6GAL1 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPKEKKKGS YYDSFKLQTK EFQVLKSLGK LAMGSDSQSV SSSSTQDPHR GRQTLGSLRG LAKAKPEASF QVWNKDSSSK NLIPRLQKIW KNYLSMNKYK VSYKGPGPGI KFSAEALRCH LRDHVNVSMV EVTDFPFNTS EWEGYLPKES IRTKAGPWGR CAVVSSAGSL KSSQLGREID DHDAVLRFNG APTANFQQDV GTKTTIRLMN SQLVTTEKRF LKDSLYNEGI LIVWDPSVYH SDIPKWYQNP DYNFFNNYKT YRKLHPNQPF YILKPQMPWE LWDILQEISP EEIQPNPPSS GMLGIIIMMT LCDQVDIYEF LPSKRKTDVC YYYQKFFDSA CTMGAYHPLL YEKNLVKHLN QGTDEDIYLL GKATLPGFRT IHCHHHHHH
• Sequence Length: 406

• Applicable Applications for ST6GAL1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ST6GAL1 recombinant protein

ST6GAL1, also known as beta-galactoside alpha-2,6-sialyltransferase 1, is a type II membrane protein localized in the trans-Golgi network and catalyzes 2,6-sialylation of Gal beta 1,4-GlcNAc structures on N-glycans. It is highly expressed in the liver and also expressed in most other tissues to some extent. Its deficiency causes abnormalities in B cell immunoreactivity. Recombinant human ST6GAL1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for ST6GAL1 recombinant protein

Enzymes & Proteases

References

Kuhn B., et al. (2013) Acta Crystallogr D Biol Crystallogr. 69:1826-1838.; Wu ZL., et al. (2011) Glycobiology. 21:727-733.

NCBI and Uniprot Product Information

• NCBI GI #: 27765091
• NCBI GeneID: 6480
• NCBI Accession #: NP_775323
• NCBI GenBank Nucleotide #: NM_173216.2
• UniProt Accession #: P15907; A8KA14; B2R513; D3DNV3
• Molecular Weight: 44.6kDa (389aa) 40-57kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: beta-galactoside alpha-2,6-sialyltransferase 1 isoform a
• NCBI Official Synonym Full Names: ST6 beta-galactoside alpha-2,6-sialyltransferase 1
• NCBI Official Symbol: ST6GAL1
• NCBI Official Synonym Symbols: ST6N; SIAT1; ST6GalI
• NCBI Protein Information: beta-galactoside alpha-2,6-sialyltransferase 1
• UniProt Protein Name: Beta-galactoside alpha-2,6-sialyltransferase 1
• Protein Family: Beta-galactoside alpha-2,6-sialyltransferase
• UniProt Gene Name: ST6GAL1
• UniProt Synonym Gene Names: SIAT1; Alpha 2,6-ST 1; ST6GalI

NCBI Description

This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017]

Uniprot Description

Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.