ST6GAL1 recombinant protein

ST6GAL1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120864

• Gene Names: ST6GAL1; ST6N; SIAT1; ST6GalI
• Purity: >95% as determined by SDS-PAGE
• Synonyms: ST6GAL1; ST6GAL1 Protein; Human; Recombinant (His Tag); Human ST6GAL1/ST6GAL-I Protein (His Tag); SIAT1 Protein; ST6GalI Protein; ST6N Protein; ST6 beta-galactosamide alpha-2; 6-sialyltranferase 1; ST6GAL1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Lys27-Cys406

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human ST6GAL1 (P15907)(Lys 27-Cys 406) was expressed, with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ST6GAL1 recombinant protein

Background: Beta-galactoside alpha-2,6-sialyltransferase 1, also known as B-cell antigen CD75, Sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, ST6GAL1 and SIAT1, is a single-pass type II membrane protein that belongs to the glycosyltransferase 29 family. Sialyltransferases are key enzymes in the biosynthesis of sialoglycoconjugates that catalyze the transfer of sialic residue from its activated form to an oligosaccharidic acceptor. ST6GAL1/SIAT1 is normally found in the Golgi but can be proteolytically processed to a soluble form. It is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. beta-Galactoside alpha2,6-sialyltransferases ST6GAL1 and ST6GAL2 are the two unique members of the ST6GAL family described in higher vertebrates. ST6GAL1/SIAT1 transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.

References

Collins,B.E. et al., 2006, Nat Immunol. 7(2):199-206. Videira,P.A. et al., 2008, Glycoconj J. 25(3): 259-68. Petit,D. et al., 2010, J Biol Chem. 285(49): 38399-414. Kroes,R.A. et al., 2010, Proc Natl Acad Sci USA.107(28):12646-51.

NCBI and Uniprot Product Information

• NCBI GI #: 4506949
• NCBI GeneID: 6480
• NCBI Accession #: NP_003023.1
• NCBI GenBank Nucleotide #: NM_003032.2
• UniProt Accession #: P15907; A8KA14; D3DNV3
• Molecular Weight: 46,605 Da
• NCBI Official Full Name: beta-galactoside alpha-2,6-sialyltransferase 1 isoform a
• NCBI Official Synonym Full Names: ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
• NCBI Official Symbol: ST6GAL1
• NCBI Official Synonym Symbols: ST6N; SIAT1; ST6GalI
• NCBI Protein Information: beta-galactoside alpha-2,6-sialyltransferase 1; ST6Gal I; alpha 2,6-ST 1; B-cell antigen CD75; sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase); CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase; CMP-N-acetylneuramin
• UniProt Protein Name: Beta-galactoside alpha-2,6-sialyltransferase 1
• Protein Family: Beta-galactoside alpha-2,6-sialyltransferase
• UniProt Gene Name: ST6GAL1
• UniProt Synonym Gene Names: SIAT1; Alpha 2,6-ST 1; ST6GalI
• UniProt Entry Name: SIAT1_HUMAN

NCBI Description

This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Three transcript variants encoding two different isoforms have been described. [provided by RefSeq, Aug 2009]

Uniprot Description

SIAT1: a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. Is normally found in the trans cisternae of the Golgi but which can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found.

Protein type: EC 2.4.99.1; Membrane protein, integral; Glycan Metabolism - N-glycan biosynthesis; Transferase

Chromosomal Location of Human Ortholog: 3q27.3

Cellular Component: Golgi membrane; integral to Golgi membrane

Molecular Function: sialyltransferase activity; beta-galactoside alpha-2,6-sialyltransferase activity

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; dolichol-linked oligosaccharide biosynthetic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; N-acetylneuraminate metabolic process; humoral immune response