Rabbit anti-Human PLA2G12A Polyclonal Antibody | anti-PLA2G12A antibody

PLA2G12A, ID (PLA2G12A, PLA2G12, Group XIIA secretory phospholipase A2, Phosphatidylcholine 2-acylhydrolase 12A) (Biotin)

Cat. Num. AAA6334511

• Gene Names: PLA2G12A; GXII; ROSSY; PLA2G12
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PLA2G12A; Polyclonal Antibody; ID (PLA2G12A; PLA2G12; Group XIIA secretory phospholipase A2; Phosphatidylcholine 2-acylhydrolase 12A) (Biotin); Phosphatidylcholine 2-acylhydrolase 12A; anti-PLA2G12A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PLA2G12A.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PLA2G12A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 55-83 in the central region of human PLA2G12A.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in A2058 cell line lysates (35ug/lane) usingMBS6005502. This demonstrates that MBS6005502 detected the PLA2G12A protein (arrow).)

Western Blot (WB)

(Western Blot analysis of PLA2G12A (arrow) in 293 cell lysates (2ug/lane) using MBS6005502 either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PLA2G12A gene.)

Related Product Information for anti-PLA2G12A antibody

Secreted phospholipase A2 (sPLA2) enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as PLA2G12A, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s (Gelb et al., 2000 [PubMed 11031251]).

Product Categories/Family for anti-PLA2G12A antibody

Antibodies; Enzymes; Phospholipase

References

1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 2. Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010). 3. Chen, W., et al. Proc. Natl. Acad. Sci. U.S.A. 107(16):7401-7406(2010). 4. Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009). 5. Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 21361944
• NCBI GeneID: 81579
• NCBI Accession #: NP_110448.2
• NCBI GenBank Nucleotide #: NM_030821.4
• UniProt Accession #: Q9BZM1; Q9BZ89
• Molecular Weight: 21,067 Da
• NCBI Official Full Name: group XIIA secretory phospholipase A2
• NCBI Official Synonym Full Names: phospholipase A2, group XIIA
• NCBI Official Symbol: PLA2G12A
• NCBI Official Synonym Symbols: GXII; ROSSY; PLA2G12
• NCBI Protein Information: group XIIA secretory phospholipase A2; GXII sPLA2; group XII secreted phospholipase A2; group XIIA secreted phospholipase A2; phosphatidylcholine 2-acylhydrolase 12A; phospholipase A2, group XII; sPLA2-XII
• UniProt Protein Name: Group XIIA secretory phospholipase A2
• Protein Family: Group XIIA secretory phospholipase
• UniProt Gene Name: PLA2G12A
• UniProt Synonym Gene Names: PLA2G12; GXII sPLA2; sPLA2-XII
• UniProt Entry Name: PG12A_HUMAN

NCBI Description

Secreted phospholipase A2 (sPLA2) enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as PLA2G12A, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s (Gelb et al., 2000 [PubMed 11031251]).[supplied by OMIM, Mar 2008]

Uniprot Description

PLA2G12A: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl- phosphatidylcholine or -phosphatidylethanolamine. Belongs to the phospholipase A2 family.

Protein type: Phospholipase; Lipid Metabolism - linoleic acid; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - ether lipid; Secreted, signal peptide; Endoplasmic reticulum; Lipid Metabolism - arachidonic acid; EC 3.1.1.4; Secreted; Lipid Metabolism - alpha-linolenic acid

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: Golgi apparatus; endoplasmic reticulum; extracellular region

Molecular Function: calcium-dependent phospholipase A2 activity; calcium ion binding

Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; lipid catabolic process