Rabbit anti-Human FLASH Polyclonal Antibody | anti-FLASH antibody

FLASH Antibody

Cat. Num. AAA9411606

• Gene Names: CASP8AP2; CED-4; FLASH; RIP25
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: FLASH; Polyclonal Antibody; FLASH Antibody; anti-FLASH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 3810

• Applicable Applications for anti-FLASH antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a synthetic peptide corresponding to amino acids near the carboxy terminus of human FLASH which differ from those of mouse by one amino acid.
• Target Name: FLASH
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of FLASH in HeLa whole cell lysate with FLASH antibody at 0.5 ug/mL.)

Immunocytochemistry (ICC)

(Immunocytochemistry of FLASH in HeLa cells with FLASH antibody at 10 ug/mL.)

Related Product Information for anti-FLASH antibody

A novel mammalian CED-4 homologous was recently identified and cloned in mouse and human and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8. There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity. FLASH is widely expressed.

Product Categories/Family for anti-FLASH antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 5533376
• NCBI GeneID: 9994
• Molecular Weight: 222,658 Da
• NCBI Official Full Name: Homo sapiens FLASH mRNA, partial cds
• NCBI Official Synonym Full Names: caspase 8 associated protein 2
• NCBI Official Symbol: CASP8AP2
• NCBI Official Synonym Symbols: CED-4; FLASH; RIP25
• NCBI Protein Information: CASP8-associated protein 2
• UniProt Protein Name: CASP8-associated protein 2
• UniProt Gene Name: CASP8AP2
• UniProt Entry Name: C8AP2_HUMAN

NCBI Description

This protein is highly similar to FLASH, a mouse apoptotic protein identified by its interaction with the death-effector domain (DED) of caspase 8. Studies of FLASH protein suggested that this protein may be a component of the death-inducing signaling complex that includes Fas receptor, Fas-binding adapter FADD, and caspase 8, and plays a regulatory role in Fas-mediated apoptosis. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Nov 2008]

Uniprot Description

CASP8AP2: Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF- kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase.

Protein type: Apoptosis; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 6q15

Cellular Component: PML body; mitochondrion; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; SUMO polymer binding; death receptor binding; apoptotic protease activator activity; caspase activator activity; transcription corepressor activity

Biological Process: caspase activation; regulation of transcription, DNA-dependent; induction of apoptosis via death domain receptors; transcription, DNA-dependent; signal transduction; cell cycle